{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:55:41Z","timestamp":1787014541618,"version":"build-2736575974"},"reference-count":101,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,4,19]],"date-time":"2012-04-19T00:00:00Z","timestamp":1334793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p>\n                    Gonadotrophin\u2010releasing hormone (GnRH) agonists are used to treat gonadal steroid\u2010dependent disorders in humans and to contracept animals. These agonists are considered to work by desensitising gonadotrophs to GnRH, thereby suppressing follicle\u2010stimulating hormone (FSH) and luteinising hormone (LH) secretion. It is not known whether changes occur in the cellular composition of the pituitary gland after chronic GnRH agonist exposure. Adult male Sprague\u2013Dawley rats were treated with a sham, deslorelin, or deslorelin plus testosterone implant for 41.0\u2003\u00b1\u20030.6\u2003days. In a second experiment, rats were castrated and treated with deslorelin and\/or testosterone. Pituitary sections were labelled immunocytochemically for FSH\u03b2 and LH\u03b2, or gonadotrophin \u03b1 subunit (\u03b1GSU). Deslorelin suppressed testis weight by two\u2010thirds and reduced plasma FSH and LH in intact rats. Deslorelin decreased the percentage of gonadotrophs, although the effect was specific to the FSH\u03b2\u2010immunoreactive (\u2010ir) cells. Testosterone did not reverse the deslorelin\u2010induced reduction in the overall gonadotroph population. However, in the presence of testosterone, the proportion of gonadotrophs that was FSH\u03b2\u2010ir increased in the remaining gonadotrophs. There was no effect of treatment on the total LH\u03b2\u2010ir cell population, although the loss of FSH\u03b2 in bi\u2010hormonal cells increased the proportion of mono\u2010hormonal LH\u03b2\u2010ir gonadotrophs. The castration\u2010induced plasma LH and FSH increases were suppressed by deslorelin, testosterone or both. Castration increased both LH\u2010ir and FSH\u2010ir without increasing the overall gonadotroph population, thus increasing the proportion of bi\u2010hormonal cells. Deslorelin suppressed these increases. Testosterone increased FSH\u2010ir in deslorelin\u2010treated castrate rats. Deslorelin did not affect \u03b1GSU immunoreactivity, suggesting that the gonadotroph population\n                    <jats:italic>per se<\/jats:italic>\n                    is not eliminated by deslorelin, although the ability of gonadotrophs to synthesise FSH\u03b2 is compromised. We hypothesise that the FSH dominant suppression may be central to the long\u2010term contraceptive efficacy of deslorelin in the male.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2826.2011.02271.x","type":"journal-article","created":{"date-parts":[[2011,12,15]],"date-time":"2011-12-15T03:32:34Z","timestamp":1323919954000},"page":"737-747","source":"Crossref","is-referenced-by-count":16,"title":["Predominant Suppression of Follicle\u2010Stimulating Hormone \u03b2\u2010Immunoreactivity after Long\u2010Term Treatment of Intact and Castrate Adult Male Rats with the Gonadotrophin\u2010Releasing Hormone Agonist\n                    <i>Deslorelin<\/i>"],"prefix":"10.1111","volume":"24","author":[{"given":"A. W.","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. S.","family":"Asa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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