{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:48:25Z","timestamp":1791035305415,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,4,19]],"date-time":"2012-04-19T00:00:00Z","timestamp":1334793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p>The mRNA expression of genes for corticotrophin\u2010releasing hormone (<jats:italic>CRH<\/jats:italic>) and the hormone receptors <jats:italic>CRH<\/jats:italic>\u2010receptor\/<jats:italic>CRH\u2010R<\/jats:italic>, glucocorticoid receptor 1\/2 (<jats:italic>GR1\/2<\/jats:italic>) and mineralocorticoid receptor (<jats:italic>MR<\/jats:italic>) was studied in the brain, pituitary and gill of tilapia (<jats:italic>Oreochromis mossambibus<\/jats:italic>) during salinity and handling stress by real\u2010time quantitative\u2010polymerase chain reaction analysis. The results indicated that the transcripts of <jats:italic>CRH<\/jats:italic> and <jats:italic>CRH\u2010R<\/jats:italic> were increased in the forebrain, midbrain and gill, whereas elevated hypothalamic <jats:italic>CRH<\/jats:italic> mRNA suppressed the <jats:italic>CRH\u2010R<\/jats:italic> mRNA in the pituitary in seawater (SW) fish. The levels of plasma osmolality and cortisol were significantly increased in SW compared to freshwater fish. The up\u2010regulation of <jats:italic>GR1<\/jats:italic>, <jats:italic>GR2<\/jats:italic>, <jats:italic>MR<\/jats:italic> and <jats:italic>\u03b1\u2010NKA<\/jats:italic> (Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\u2010ATPase) transcripts in SW fish provided evidence that cortisol responds to stress and involves ion\u2010base regulation via the <jats:italic>GR1<\/jats:italic>, <jats:italic>GR2<\/jats:italic> and <jats:italic>MR<\/jats:italic> receptors in the gill. These data suggest that GR1, GR2 and MR have a pivotal role in the brain and gill. <jats:italic>GR1<\/jats:italic>, <jats:italic>GR2<\/jats:italic> and <jats:italic>MR<\/jats:italic> expression may be dependent on CRH and cortisol expression in the brain and gill. In addition, we performed <jats:italic>in situ<\/jats:italic> hybridisation analysis to localise and differentiate the <jats:italic>CRH<\/jats:italic>, <jats:italic>CRH\u2010R<\/jats:italic>, <jats:italic>GR1<\/jats:italic>, <jats:italic>GR2<\/jats:italic> and <jats:italic>MR<\/jats:italic> transcripts in the brain of FW\u2010 and SW\u2010acute acclimated tilapia during salinity stress. In almost all transcripts, the hybridisation signal was significantly abundant in the SW\u2010acute acclimated tilapia brain, especially in the dorsal ventral cephalon, dorsal nucleus preopticus pars magnocellularis and dorsal nucleus preopticus pars parvocellularis. Salinity stress induced differential and specific responses in the gill and brain compared to handling stress.<\/jats:p>","DOI":"10.1111\/j.1365-2826.2012.02282.x","type":"journal-article","created":{"date-parts":[[2012,1,18]],"date-time":"2012-01-18T00:19:27Z","timestamp":1326845967000},"page":"818-830","source":"Crossref","is-referenced-by-count":39,"title":["Involvement of Corticotrophin\u2010Releasing Hormone and Corticosteroid Receptors in the Brain\u2013Pituitary\u2013Gill of Tilapia During the Course of Seawater Acclimation"],"prefix":"10.1111","volume":"24","author":[{"given":"A.","family":"Aruna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Nagarajan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.\u2010F.","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,4,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2006.01.015"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.091553298"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1123348"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2008.03.024"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-3057(02)00791-8"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0566"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0310141"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1677\/JOE-08-0100"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2826.2003.01099.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00677-4"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2826.2003.01100.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0320627"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10377"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21496"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.00701"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1546-5098(08)60250-2"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00347.2004"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2008.12.003"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2011.03.021"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.514"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.00895"},{"key":"e_1_2_6_23_2","first-page":"1","volume-title":"World Aquaculture","author":"Costa\u2010Pierce BA","year":"2000"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.17.149"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jembe.2007.05.005"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jembe.2009.05.004"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.1983.tb02928.x"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.12.012"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2004.04.005"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2010.07.009"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.106.1.255"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1668\/0003-1569(2001)041[0781:ECOOIT]2.0.CO;2"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1364"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00050.2003"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.002873"},{"key":"e_1_2_6_36_2","first-page":"R101","article-title":"Synthesis of gill Na+\u2010K+\u2010ATPase in Atlantic salmon smolts: differences in \u03b1\u2010mRNA and \u03b1\u2010protein levels","volume":"278","author":"D\u2019Cotta H","year":"2000","journal-title":"Am J Physiol"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00054.2004"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.06142"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812062106"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-03-00585.1991"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.1994.tb00636.x"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1159\/000054646"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.50.21"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2005.04.065"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01973"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1390"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-0128"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1765"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1998.7182"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1997.77.3.591"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(99)00262-2"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1997.6955"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00448.2002"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2005.09.050"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1086\/317737"},{"key":"e_1_2_6_56_2","doi-asserted-by":"crossref","first-page":"3953","DOI":"10.1242\/jeb.204.22.3953","article-title":"Evidence for a mineralocorticoid like receptor linked to branchial chloride cell proliferation in freshwater rainbow trout","volume":"204","author":"Sloman KA","year":"2001","journal-title":"J Exp Biol"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1997.7019"}],"container-title":["Journal of Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2826.2012.02282.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2826.2012.02282.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T10:27:56Z","timestamp":1697365676000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2826.2012.02282.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4,19]]},"references-count":56,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1111\/j.1365-2826.2012.02282.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2826.2012.02282.x","archive":["Portico"],"relation":{},"ISSN":["0953-8194","1365-2826"],"issn-type":[{"value":"0953-8194","type":"print"},{"value":"1365-2826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4,19]]}}}