{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:02:12Z","timestamp":1760781732934,"version":"3.40.3"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T00:00:00Z","timestamp":1350345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:p>Parkinson\u2019s disease (PD) is characterised by the loss of nigrostriatal dopamine (DA) neurones and glutamate overactivity. There is substantial evidence to suggest that oestrogens prevent or delay the disease. 17\u03b2\u2010oestradiol has neuroprotective effects in the 1\u2010methyl\u20104\u2010phenyl\u20101,2,3,6\u2010tetrahydropyridine (MPTP) mouse model of PD and modulates brain NMDA receptors. In MPTP\u2010lesioned mice, oestrogen receptor (ER)\u03b1 and ER\u03b2 are important in 17\u03b2\u2010oestradiol\u2010induced neuroprotection. To evaluate the role of ERs in the response of NMDA receptors to lesion, we compared wild\u2010type (WT) with ER knockout (KO) C57Bl\/6 male mice that received 7, 9 or 11\u2003mg\/kg of MPTP. These mice were also treated with MPTP (9\u2003mg\/kg) and 17\u03b2\u2010oestradiol. [<jats:sup>3<\/jats:sup>H]Ro 25\u20106981 specific binding autoradiography was used to label NMDA receptors containing NR2B subunits. In the frontal and cingulate cortex and striatum, vehicle\u2010treated WT mice had higher [<jats:sup>3<\/jats:sup>H]Ro 25\u20106981 specific binding compared to ERKO mice. Cortical [<jats:sup>3<\/jats:sup>H]Ro 25\u20106981 specific binding decreased with increasing doses of MPTP in WT and ERKO\u03b1 but not ERKO\u03b2 mice, whereas a dose\u2010related decrease was only observed in the striatum of WT mice remaining low in ERKO\u03b1 and ERKO\u03b2 mice. No effect of 17\u03b2\u2010oestradiol treatment in intact or MPTP\u2010lesioned mice of all three genotypes was observed in the cortex, whereas it increased striatal specific binding of intact ERKO\u03b2 and MPTP\u2010lesioned WT mice. Striatal [<jats:sup>3<\/jats:sup>H]Ro 25\u20106981 specific binding positively correlated with striatal DA concentrations only in WT mice. MPTP and 17\u03b2\u2010oestradiol treatments had more limited effects in the hippocampus. Only in the CA3 and dentate gyrus did vehicle and 17\u03b2\u2010oestradiol\u2010treated ERKO\u03b1 mice have higher [<jats:sup>3<\/jats:sup>H]Ro 25\u20106981 specific binding than WT and ERKO\u03b2 mice, whereas MPTP decreased this specific binding only in the CA1, CA2 and CA3 of ERKO\u03b1 mice. Hence, brain NMDA receptors were affected by the deletion of ERs, which affect the response to MPTP and 17\u03b2\u2010oestradiol treatments with brain region specificity.<\/jats:p>","DOI":"10.1111\/j.1365-2826.2012.02349.x","type":"journal-article","created":{"date-parts":[[2012,6,6]],"date-time":"2012-06-06T14:49:41Z","timestamp":1338994181000},"page":"1375-1385","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Oestrogen Receptors on Brain NMDA Receptors of 1\u2010Methyl\u20104\u2010Phenyl\u20101,2,3,6\u2010Tetrahydropyridine Mice"],"prefix":"10.1111","volume":"24","author":[{"given":"S.","family":"Al\u2010Sweidi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Morissette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Di 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