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The drug\u2010transporting isoform of P\u2010gp is coded by a single human gene, ABCB1, and shares 80% homology with the murine drug\u2010transporting isoforms, abcb1a and abcb1b, which share 92% homology with each other. Although these murine isoforms are highly similar, there are known affinity differences between the isoforms, and the localisation of the two isoforms in the brain is also disputed. Studies using mice genetically modified to be deficient in one or both isoforms of P\u2010gp have also resulted in conflicting data. The contribution of the abcb1a isoform, which is considered to contribute most to the central nervous system (CNS)\u2010protective role of P\u2010gp, is investigated in the present study using CF\u20101\u2010abcb1a(\u2212\/\u2212) mice and the well\u2010established brain\/choroid plexus perfusion technique. Twenty\u2010minute <jats:italic>in situ<\/jats:italic> brain\/choroid plexus perfusions in CF\u20101\u2010abcb1a(\u2212\/\u2212) mice indicated the increased accumulation of [<jats:sup>3<\/jats:sup>H]cortisol, [<jats:sup>3<\/jats:sup>H]corticosterone and [<jats:sup>3<\/jats:sup>H]dexamethasone in most of the brain regions examined compared to CF\u20101\u2010abcb1a(+\/+) mice. Taken together with our earlier published studies in abcb1a\/b(\u2212\/\u2212) mice, these data strongly suggest that the <jats:italic>in vivo<\/jats:italic> CNS accumulation of glucocorticoids obtained using single knockout strains [e.g. abcb1a(\u2212\/\u2212)] cannot be directly compared with those obtained in double knockout strains [e.g. abcb1a\/b(\u2212\/\u2212)].<\/jats:p>","DOI":"10.1111\/j.1365-2826.2012.02353.x","type":"journal-article","created":{"date-parts":[[2012,6,16]],"date-time":"2012-06-16T03:08:49Z","timestamp":1339816129000},"page":"1440-1446","source":"Crossref","is-referenced-by-count":11,"title":["Changes in the Brain Accumulation of Glucocorticoids in abcb1a\u2010Deficient CF\u20101 Mice"],"prefix":"10.1111","volume":"24","author":[{"given":"B. L.","family":"Mason","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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