{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:02:22Z","timestamp":1791190942404,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,11,20]],"date-time":"2012-11-20T00:00:00Z","timestamp":1353369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) are short (\u223c22 nucleotides) noncoding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> molecules that post\u2010transcriptionally repress the expression of protein\u2010coding genes by binding to 3\u2032\u2010untranslated regions of the target m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s. To identify mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s selectively expressed within the hypothalamus, a part of the brain that controls vital bodily functions, we employed locked nucleic acid (<jats:styled-content style=\"fixed-case\">LNA<\/jats:styled-content>)\u2010fluorescent <jats:italic>in situ<\/jats:italic> hybridisation (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>). The expression pattern of the mature mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107a, mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107b, mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u2010137 and mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u2010153 in mouse brain tissue sections was investigated. Although all studied mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were present in the hypothalamus, mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107a, was the only mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> found to be enriched in the hypothalamus, with low or no expression in other parts of the central nervous system (<jats:styled-content style=\"fixed-case\">CNS<\/jats:styled-content>). Within the hypothalamus, strong mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107a expression was distinct and restricted to some hypothalamic nuclei and adjacent areas. mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107a expression was particularly prominent in the subfornical organ, as well as the suprachiasmatic, paraventricular, periventricular, supraoptic, dorsomedial and arcuate nuclei. Identical expression patterns for mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107a were seen in mouse and rat hypothalamus. By combining <jats:styled-content style=\"fixed-case\">LNA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content> with immunohistochemistry, it was shown that mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107a was preferentially present in small orexigenic neuropeptide <jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>\/agouti\u2010related protein\u2010containing\u2010neurones located in the ventromedial aspect of the arcuate nucleus but not in large pro\u2010opiomelanocortin\/cocaine\u2010 and amphetamine\u2010regulated transcript\u2010containing anorexigenic neurones of the ventrolateral part of the arcuate nucleus. The limited and distinct expression of mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107a in the <jats:styled-content style=\"fixed-case\">CNS<\/jats:styled-content> suggests that mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107a has a role in post\u2010transcriptional regulation in hypothalamic neurones. Particularly strong expression of mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107a in neurones located in the ventromedial division of the arcuate nucleus, a subregion with a weak blood\u2013brain barrier, raises the possibility that mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u20107a is influenced by circulating hormones and is a regulator of the genes involved in body weight control.<\/jats:p>","DOI":"10.1111\/j.1365-2826.2012.02358.x","type":"journal-article","created":{"date-parts":[[2012,7,9]],"date-time":"2012-07-09T05:21:27Z","timestamp":1341811287000},"page":"1492-1504","source":"Crossref","is-referenced-by-count":50,"title":["Locked Nucleic Acid\u2010Based <i>In Situ<\/i> Hybridisation Reveals miR\u20107a as a Hypothalamus\u2010Enriched Micro<scp>RNA<\/scp> with a Distinct Expression Pattern"],"prefix":"10.1111","volume":"24","author":[{"given":"S.","family":"Herzer","sequence":"first","affiliation":[{"name":"Department of Neuroscience Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Silahtaroglu","sequence":"additional","affiliation":[{"name":"Wilhelm Johannsen Centre for Functional Genome Research Institute for Cellular and Molecular Medicine University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Meister","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Karolinska Institutet Stockholm 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