{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:06:00Z","timestamp":1700697960616},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,12,21]],"date-time":"2012-12-21T00:00:00Z","timestamp":1356048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Neuroendocrinology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"The present study demonstrates that a significant proportion of high voltage\u2010activated (HVA<\/jats:styled-content>) C<\/jats:styled-content>a2+<\/jats:sup> influx in native rat anterior pituitary cells is carried through non\u2010L<\/jats:styled-content>\u2010type C<\/jats:styled-content>a2+<\/jats:sup> channels. Using whole\u2010cell patch\u2010clamp recordings and specific C<\/jats:styled-content>a2+<\/jats:sup> channel toxin blockers, we show that approximately 35% of the HVA C<\/jats:styled-content>a2+<\/jats:sup> influx in somatotrophs and lactotrophs is carried through C<\/jats:styled-content>av<\/jats:sub>2.1, C<\/jats:styled-content>av<\/jats:sub>2.2 and C<\/jats:styled-content>av<\/jats:sub>2.3 channels, and that somatotrophs and lactotrophs share similar proportions of these non\u2010L<\/jats:styled-content>\u2010type C<\/jats:styled-content>a2+<\/jats:sup> channels. Furthermore, experiments on mixed populations of native anterior pituitary cells revealed that the fraction of HVA C<\/jats:styled-content>a2+<\/jats:sup> influx carried through these non\u2010L<\/jats:styled-content>\u2010type C<\/jats:styled-content>a2+<\/jats:sup> channels might even be higher (approximately 46%), suggesting that non\u2010L<\/jats:styled-content>\u2010type channels exist in the majority of native anterior pituitary cells. Using western blotting, immunoblots for \u03b11C<\/jats:sub>, \u03b11D<\/jats:sub>, \u03b11A<\/jats:sub>, \u03b11B<\/jats:sub> and \u03b11E<\/jats:sub> C<\/jats:styled-content>a2+<\/jats:sup> channel subunits were identified in native rat anterior pituitary cells. Additionally, using reverse transcriptase\u2010polymerase chain reaction, cDNA<\/jats:styled-content> transcripts for \u03b11C<\/jats:sub>, \u03b11D<\/jats:sub>, \u03b11A<\/jats:sub> and \u03b11B<\/jats:sub> C<\/jats:styled-content>a2+<\/jats:sup> channel subunits were identified. Transcripts for \u03b11E<\/jats:sub> were nonspecific and transcripts for \u03b11S<\/jats:sub> were not detected at all (control). Taken together, these results clearly demonstrate the existence of multiple HVA C<\/jats:styled-content>a2+<\/jats:sup> channels in the membrane of rat native anterior pituitary cells. Whether these channels are segregated among different membrane compartments was investigated further in flotation assays, demonstrating that C<\/jats:styled-content>av<\/jats:sub>2.1, C<\/jats:styled-content>av<\/jats:sub>1.2 and caveolin\u20101 were mostly localised in light fractions of N<\/jats:styled-content>ycodenz gradients (i.e. in lipid raft domains). C<\/jats:styled-content>av<\/jats:sub>1.3 channels were distributed among both light and heavy fractions of the gradients (i.e. among raft and nonraft domains), whereas C<\/jats:styled-content>av<\/jats:sub>2.2 and C<\/jats:styled-content>av<\/jats:sub>2.3 channels were distributed mostly among nonraft domains. In summary, in the present study, we demonstrate multiple pathways for HVA C<\/jats:styled-content>a2+<\/jats:sup> influx through L<\/jats:styled-content>\u2010type and non\u2010L<\/jats:styled-content>\u2010type C<\/jats:styled-content>a2+<\/jats:sup> channels in the membrane of native anterior pituitary cells. The compartmentalisation of these channels among raft and nonraft membrane domains might be essential for their proper regulation by separate receptors and signalling pathways.<\/jats:p>","DOI":"10.1111\/j.1365-2826.2012.02372.x","type":"journal-article","created":{"date-parts":[[2012,8,10]],"date-time":"2012-08-10T11:52:13Z","timestamp":1344599533000},"page":"76-86","source":"Crossref","is-referenced-by-count":3,"title":["Multiple Pathways for High Voltage\u2010Activated C<\/scp>a2+<\/sup> Influx in Anterior Pituitary Lactotrophs and Somatotrophs"],"prefix":"10.1111","volume":"25","author":[{"given":"A.","family":"Tzour","sequence":"first","affiliation":[{"name":"Department of Medical Neurobiology Institute for Medical Research Israel\u2013Canada Hebrew University Faculty of Medicine Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Sosial","sequence":"additional","affiliation":[{"name":"Department of Medical Neurobiology Institute for Medical Research Israel\u2013Canada Hebrew University Faculty of Medicine Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Meir","sequence":"additional","affiliation":[{"name":"Minerva Center for Calcium and Bone Metabolism Hadassah University Hospital Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Canello","sequence":"additional","affiliation":[{"name":"Department of Neurology The Agnes Ginges Center for Human Neurogenetics Hadassah University Hospital Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Naveh\u2010Many","sequence":"additional","affiliation":[{"name":"Minerva Center for Calcium and Bone Metabolism Hadassah University Hospital Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Gabizon","sequence":"additional","affiliation":[{"name":"Department of Neurology The Agnes Ginges Center for Human Neurogenetics Hadassah University Hospital Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Nussinovitch","sequence":"additional","affiliation":[{"name":"Department of Medical Neurobiology Institute for Medical Research Israel\u2013Canada Hebrew University Faculty of Medicine Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp016920"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-13-2-256"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000054360"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00727-0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2005.03.003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.15.7.1222"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105386200"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1989.tb24124.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-123-1-611"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)91610-7"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018090"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2010-0005"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2011.11.003"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2011.12.005"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.136.9.3916"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.137.4.1429"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240100567"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103724200"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2007.01596.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00101.2003"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00118.2005"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020109"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.10.6324"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90223-Z"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/355827a0"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90185-T"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.22.10576"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900327"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.02.004"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/8070"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00317a001"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.12.4327"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90191-8"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00580957"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(97)00086-5"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi981255g"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-21-09235.1999"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(01)75681-0"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(03)02084-3"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.058271"},{"key":"e_1_2_7_42_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2826.2007.01616.x","article-title":"Paracrinicity: the story of 30\u00a0years of cellular pituitary crosstalk","volume":"20","author":"Denef C","year":"2008","journal-title":"J Neuroendocrinol"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2570274.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2002.1179"},{"key":"e_1_2_7_45_1","doi-asserted-by":"crossref","first-page":"3345","DOI":"10.1523\/JNEUROSCI.19-09-03345.1999","article-title":"Tonic dopamine inhibition of L\u2010type Ca2+ channel activity reduces alpha1D Ca2+ channel gene expression","volume":"19","author":"Fass DM","year":"1999","journal-title":"J Neurosci"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.48.121506.124841"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.191585"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.16.1.521"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503465103"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2004.05.012"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2010.04.006"}],"container-title":["Journal of Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2826.2012.02372.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2826.2012.02372.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T14:59:35Z","timestamp":1700665175000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2826.2012.02372.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,21]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1111\/j.1365-2826.2012.02372.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2826.2012.02372.x","archive":["Portico"],"relation":{},"ISSN":["0953-8194","1365-2826"],"issn-type":[{"value":"0953-8194","type":"print"},{"value":"1365-2826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,21]]}}}