{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:22:39Z","timestamp":1762078959874,"version":"3.33.0"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,9,3]],"date-time":"2007-09-03T00:00:00Z","timestamp":1188777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Pharm &amp; Therapeutics"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p>In recurrent airway obstruction (RAO) or heaves, bronchospasm has been attributed to enhanced cholinergic activity. However, the expression and function of muscarinic acetylcholine receptors (mAChR) and their signaling components are not yet known. Thus, we examined the expression, subtype distribution and postreceptor signaling pathways of mAChR in the peripheral lung, bronchial and tracheal epithelia with the underlying smooth muscle from nine horses with RAO and 11 healthy control horses. In RAO horses, no significant segment\u2010dependent alteration in mAChR density and subtype distribution (assessed by [<jats:italic>N<\/jats:italic>\u2010methyl\u2010<jats:sup>3<\/jats:sup>H]\u2010scopolamine binding; ([<jats:sup>3<\/jats:sup>H]\u2010NMS)), was found, except a trend in receptor down\u2010regulation in some peripheral parts of the lung. The total number of high mAChR binding sites (assessed by carbachol\u2010displacement experiments in the presence or absence of guanosine 5\u2032\u2010triphosphate) was not changed in RAO, suggesting that the functional coupling of mAChR to the corresponding G\u2010proteins is intact. The M<jats:sub>2<\/jats:sub>\u2010mediated inhibition of adenylate cyclase (AC) as well as the M<jats:sub>3<\/jats:sub>\u2010receptor\u2010G<jats:sub>q\/11<\/jats:sub>\u2010phospholipase C (PLC) activity was not different between RAO and control airway tissues. In conclusion, in equine RAO airways, mAChR expression and function were not altered, and thus appear not to account for the enhanced cholinergic activity in RAO.<\/jats:p>","DOI":"10.1111\/j.1365-2885.2007.00897.x","type":"journal-article","created":{"date-parts":[[2007,9,7]],"date-time":"2007-09-07T09:15:52Z","timestamp":1189156552000},"page":"401-409","source":"Crossref","is-referenced-by-count":8,"title":["Equine recurrent airway obstruction does not alter airway muscarinic acetylcholine receptor expression and subtype distribution"],"prefix":"10.1111","volume":"30","author":[{"given":"G.","family":"ABRAHAM","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"KOTTKE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. 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