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Assays for viral binding and entry were performed by culturing a hepatoblastoma cell line, HepG2, and HBV particles derived from the HBV carrier in the presence or absence of neuraminidase (NA). Viral binding and entry were clearly enhanced in the presence of NA, and the enhancement of the binding could be blocked by asialo\u2010fetuin and ethylenediamine\u2010tetraacetic acid (EDTA). In addition, covalently closed circular (CCC)\u2010DNA, as a marker of infectivity, was detected in the presence of NA, but not in its absence. The optimal concentration of NA raised infectivity more than 1000 times. We concluded that this method makes it feasible to evaluate the infectivity of HBV <jats:italic>in vitro<\/jats:italic> and that ASGP\u2010R may be a specific HBV receptor once viral particles are desialylated.<\/jats:p>","DOI":"10.1111\/j.1365-2893.2005.00648.x","type":"journal-article","created":{"date-parts":[[2005,12,19]],"date-time":"2005-12-19T12:26:38Z","timestamp":1134995198000},"page":"11-18","source":"Crossref","is-referenced-by-count":22,"title":["Interaction between desialylated hepatitis B virus and asialoglycoprotein receptor on hepatocytes may be indispensable for viral binding and 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