{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T17:08:07Z","timestamp":1722445687805},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,2,20]],"date-time":"2006-02-20T00:00:00Z","timestamp":1140393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:p><jats:bold>Summary.\u2002<\/jats:bold> Murine cytomegalovirus (MCMV) has provided useful models for acute, chronic and latent CMV infection because of its similarities in structure and biology with human CMV. We report the induction of acute MCMV hepatitis with different bacterial artificial chromosome (BAC)\u2010cloned virus constructs [MCMV\u2010SEAP which includes the gene for secreted alkaline phosphatase (SEAP) under Rous sarcoma virus (RSV)\u2010promoter control, MCMV\u2010GFP which includes the gene for enhanced green fluorescent protein (eGFP) under HCMV\u2010ie promoter control, MCMV\u2010HBs includes the gene for hepatitis B surface antigen (HBsAg) under simian virus (SV)40\u2010promoter control and the DeltaMC95.21 virus in which the <jats:italic>m152<\/jats:italic> gene was deleted and substituted by the reporter gene <jats:italic>lacZ<\/jats:italic>] in order to elucidate the histopathological changes together with different reporter\u2010gene products in the liver tissue and the effect of the deletion of a certain gene. All the virus constructs induced a similar mild acute hepatitis which had its climax from days 3 to 5 post\u2010infection in immunocompetent mice. <jats:italic>In situ<\/jats:italic>, the reporter\u2010gene products beta\u2010galactosidase and secreted alkaline phosphatase could be visualized in relation to the inflammatory changes. The composition of the invading cell populations did not change even in the absence of the <jats:italic>m152<\/jats:italic> gene. Additionally discrete inflammatory changes were seen in kidney and serosa while the other organs were not involved. This model helps us understand the immunological and histopathological mechanisms of the CMV\u2010induced hepatitis, which plays an important role especially in the immunocompromised patient. The morphological changes can be analysed while the respective reporter gene product is expressed by the virus construct.<\/jats:p>","DOI":"10.1111\/j.1365-2893.2005.00696.x","type":"journal-article","created":{"date-parts":[[2006,2,20]],"date-time":"2006-02-20T16:56:41Z","timestamp":1140454601000},"page":"363-370","source":"Crossref","is-referenced-by-count":1,"title":["Induction of early murine cytomegalovirus infection by different reporter gene\u2010associated recombinant viruses"],"prefix":"10.1111","volume":"13","author":[{"given":"U.","family":"Drebber","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Haferkamp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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