{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T07:49:34Z","timestamp":1721029774821},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2006,8,2]],"date-time":"2006-08-02T00:00:00Z","timestamp":1154476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p><jats:bold>Summary.\u2002<\/jats:bold> Sporadic and epidemic acute viral hepatitis E in many developing countries is caused by hepatitis E virus (HEV). The HEV genome has been classified into three major genotypes. However, extensive diversity has been noted among HEV isolates from patients with acute hepatitis in China and Taiwan. Some reports indicated that multiple genotypes of HEV could cocirculate in the same area; even distinct genotypes of HEV could exist in the same patient. Pakistan is a highly endemic area for hepatitis E. So far only two Pakistan HEV isolates Sar\u201055 (87\u2010Pakistan\u2010A) and Abb\u20102B (88\u2010Pakistan\u20102B) have been characterized, and the nucleotide sequences of these two HEV isolates show only 90% homology. In this study, a third HEV isolate from Pakistan (87\u2010Pakistan\u2010B) is reported. The sequences of a 438\u2010bp fragment from ORF\u20102 and a 259\u2010bp fragment from the ORF\u20101\u20133 region of this new HEV isolate were obtained and sequenced. The sequence analysis showed that this new HEV isolate was very closely related to the Sar\u201055 but different from the Abb\u20102B HEV isolate. These results indicated that the Sar\u201055 (87\u2010Pakistan\u2010A) genotype is the main endemic HEV strain in the Sargodha area. These data will be useful for HEV epidemiological studies, diagnosis and vaccine development.<\/jats:p>","DOI":"10.1111\/j.1365-2893.2006.00774.x","type":"journal-article","created":{"date-parts":[[2006,11,14]],"date-time":"2006-11-14T11:20:36Z","timestamp":1163503236000},"page":"840-844","source":"Crossref","is-referenced-by-count":4,"title":["Molecular detection and sequence analysis of a new hepatitis E virus isolate from Pakistan"],"prefix":"10.1111","volume":"13","author":[{"given":"J.","family":"He","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,2]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.1999.01916.x"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199701)51:1<64::AID-JMV10>3.0.CO;2-4"},{"key":"e_1_2_7_4_2","first-page":"P637","volume-title":"Non\u2010a\/Non\u2010b Epidemic Viral Hepatitis in Algeria. 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