{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:16:48Z","timestamp":1791040608963,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2007,1,3]],"date-time":"2007-01-03T00:00:00Z","timestamp":1167782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p><jats:bold>Summary.\u2002<\/jats:bold>The mechanisms by which alcohol exacerbates liver injury in patients with hepatitis C are unknown. We used the hepatitis C virus (HCV) subgenomic replicon cell system to evaluate the effect of ethanol on HCV replication and viral protein synthesis. Our results demonstrate that alcohol stimulates HCV replicon expression at both HCV\u2010RNA and protein levels. Furthermore, we observed that ethanol treatment showed an additive effect in cyclooxygenase\u20102 (COX\u20102) protein expression and activity already induced by HCV viral proteins, and in turn increased HCV viral expression. Our results suggest that COX\u20102 activity is involved in ethanol\u2010induced HCV\u2010RNA and NS5A protein expression, because acetylsalicylic acid (ASA), a COX\u20101\/2 inhibitor, blocked this induction and downregulated COX\u20102 protein expression and activity. Therefore, we suggest that ethanol increases HCV replication expression, at least in part, by upregulating a key cellular regulator of oxidative stress pathway known as COX\u20102 or its products.<\/jats:p>","DOI":"10.1111\/j.1365-2893.2006.00837.x","type":"journal-article","created":{"date-parts":[[2007,1,4]],"date-time":"2007-01-04T13:47:57Z","timestamp":1167918477000},"page":"608-617","source":"Crossref","is-referenced-by-count":25,"title":["Additive effect of ethanol and HCV subgenomic replicon expression on COX\u20102 protein levels and activity"],"prefix":"10.1111","volume":"14","author":[{"given":"K.","family":"Trujillo\u2010Murillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"\u00c1lvarez\u2010Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Garza\u2010Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Mart\u00ednez\u2010Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Bosques\u2010Padilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ramos\u2010Jim\u00e9nez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Barrera\u2010Salda\u00f1a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. R.","family":"Rinc\u00f3n\u2010S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. M.","family":"Rivas\u2010Estilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,3]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2000-9506"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000939"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-3542(01)00164-4"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(02)00341-7"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(96)80284-4"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2003.50295"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0741-8329(02)00201-X"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2003.50275"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(02)01300-X"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5424.110"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/088282403771926283"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052713799"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001381200"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.21.8122"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.2.725"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.21.10637-10653.2002"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510270635"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510280330"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-134-2-200101160-00012"},{"key":"e_1_2_7_21_2","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S0022-3565(24)37798-5","article-title":"Differential effect of ethanol on PC12 cell death","volume":"287","author":"Oberdoerster J","year":"1998","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1225"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1997.v112.pm9041257"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2000.19621"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-2-89"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.15.9725-9734.2005"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.10.045"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.12.7648-7657.2005"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1086\/429301"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.18.8516-8523.2001"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199903113401009"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.36.040196.001153"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.3690"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(03)00414-8"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1995.1400"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2001.tb02259.x"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s000110050605"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324143"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.83.2.210"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1626"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1993.tb05234.x"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.3109\/01902149809046050"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.1.192-198.2001"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.42.27306"}],"container-title":["Journal of Viral Hepatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2893.2006.00837.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2893.2006.00837.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T19:50:25Z","timestamp":1736711425000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2893.2006.00837.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1,3]]},"references-count":43,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1111\/j.1365-2893.2006.00837.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2893.2006.00837.x","archive":["Portico"],"relation":{},"ISSN":["1352-0504","1365-2893"],"issn-type":[{"value":"1352-0504","type":"print"},{"value":"1365-2893","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1,3]]}}}