{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T06:34:32Z","timestamp":1759991672729},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,8,28]],"date-time":"2008-08-28T00:00:00Z","timestamp":1219881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Viral Hepatitis"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:p><jats:bold>Summary.\u2002<\/jats:bold> Extrahepatic replication may have important implications for the treatment of hepatitis C virus (HCV). Our aim was to analyse the association between the presence of positive\/negative strand HCV RNA in different peripheral blood cell subsets at the end of PegIFN\/RBV treatment, and treatment response in HIV\u2010coinfected patients. Thirty\u2010four HCV\u2013HIV coinfected patients who concluded 48\u2003weeks of PegIFN\/RBV treatment were included in the present study. Positive\/negative strand HCV RNA was detected by amplification of the 5\u2032 untranslated region (5\u2032 UTR) using high\u2010temperature RT\u2010PCR in immunomagnetic\u2010isolated cell subsets. Twenty\u2010three patients (67.6%) had sustained virologic response (SVR) while 11 patients (32.4%) relapsed. The frequency of positive\/negative strand HCV RNA in any cell subsets was significantly lower in patients with SVR (8.6%) compared to relapsers (63.6%) (<jats:italic>P\u2003<\/jats:italic>=\u20030.002). Baseline HCV viral load was statistically higher among patients who relapsed (<jats:italic>P\u2003<\/jats:italic>=\u20030.008), while patients with SVR had very early virologic response more frequently (<jats:italic>P<\/jats:italic>\u2003=\u20030.003). Multivariate analysis showed, among these three variables, that only the presence of positive\/negative strand HCV RNA was independently associated with relapse [<jats:italic>P\u2003<\/jats:italic>=\u20030.024; OR 14 (14\u2013137)]. In conclusion, the presence of positive\/negative strand HCV RNA at the end of treatment is associated with relapse among HCV\u2013HIV coinfected patients and might have important implications in the clinical practice.<\/jats:p>","DOI":"10.1111\/j.1365-2893.2008.01043.x","type":"journal-article","created":{"date-parts":[[2008,8,28]],"date-time":"2008-08-28T04:06:37Z","timestamp":1219896397000},"page":"21-27","source":"Crossref","is-referenced-by-count":11,"title":["HCV RNA in peripheral blood cell subsets in HCV\u2013HIV coinfected patients at the end of PegIFN\/RBV treatment is associated with virologic relapse"],"prefix":"10.1111","volume":"16","author":[{"given":"B.","family":"De Felipe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Leal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Soriano\u2010Sarabia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"L\u00f3pez\u2010Cort\u00e9s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Molina\u2010Pinelo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Vallejo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,18]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.162.18.2125"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1413-86702006000100004"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200409030-00002"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.36226"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032653"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040842"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.292.23.2839"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2006.09.009"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa020047"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200409030-00003"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.283.1.74"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.aids.0000192085.87302.39"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/509897"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/430949"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2004.057281"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/323391"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/375350"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-08-2923"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20518"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1086\/315283"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21283"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/508198"},{"key":"e_1_2_7_24_2","first-page":"550","article-title":"Clearance of HCV RNA in peripheral blood mononuclear cell as a predictor of response to antiviral therapy in patients with chronic hepatitis C","volume":"4","author":"Xu DZ","year":"2005","journal-title":"Hepatobiliary Pancreat Dis Int"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0b013e318061b5d9"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1996.43747.x"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20506"}],"container-title":["Journal of Viral Hepatitis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2893.2008.01043.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2893.2008.01043.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T22:56:58Z","timestamp":1697065018000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2893.2008.01043.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12,18]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1111\/j.1365-2893.2008.01043.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2893.2008.01043.x","archive":["Portico"],"relation":{},"ISSN":["1352-0504","1365-2893"],"issn-type":[{"value":"1352-0504","type":"print"},{"value":"1365-2893","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12,18]]}}}