{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T08:26:39Z","timestamp":1777710399214,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,4,10]],"date-time":"2012-04-10T00:00:00Z","timestamp":1334016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:p>\n                    In the last 5 years, there has been only one reported human case of West Nile virus (WNV) disease in northern Mexico. To determine if the virus was still circulating in this region, equine and entomological surveillance for WNV was conducted in the state of Nuevo Leon in northern Mexico in 2006 and 2007. A total of 203 horses were serologically assayed for antibodies to WNV using an epitope\u2010blocking enzyme\u2010linked immunosorbent assay (bELISA). Seroprevalences for WNV in horses sampled in 2006 and 2007 were 26% and 45%, respectively. Mosquito collections in 2007 produced 7365 specimens representing 15 species.\n                    <jats:italic>Culex<\/jats:italic>\n                    mosquitoes were screened for WNV RNA and other genera (\n                    <jats:italic>Mansonia, Anopheles, Aedes, Psorophora<\/jats:italic>\n                    and\n                    <jats:italic>Uranotaenia<\/jats:italic>\n                    ) were screened for flaviviruses using reverse\u2010transcription (RT)\u2010PCR. Two pools consisting of\n                    <jats:italic>Culex<\/jats:italic>\n                    spp. mosquitoes contained WNV RNA. Molecular species identification revealed that neither pool included\n                    <jats:italic>Culex quinquefasciatus<\/jats:italic>\n                    (Say) (Diptera:Culicidae) complex mosquitoes. No evidence of flaviviruses was found in the other mosquito genera examined. These data provide evidence that WNV is currently circulating in northern Mexico and that non\u2010\n                    <jats:italic>Cx. quinquefasciatus spp.<\/jats:italic>\n                    mosquitoes may be participating in the WNV transmission cycle in this region.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2915.2012.01014.x","type":"journal-article","created":{"date-parts":[[2012,4,10]],"date-time":"2012-04-10T22:11:23Z","timestamp":1334095883000},"page":"351-354","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Detection of West Nile virus\u2010specific antibodies and nucleic acid in horses and mosquitoes, respectively, in Nuevo Leon State, northern Mexico, 2006\u20132007"],"prefix":"10.1111","volume":"26","author":[{"given":"L.","family":"IBARRA\u2010JUAREZ","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"EISEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. G.","family":"BOLLING","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. J.","family":"BEATY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. J.","family":"BLITVICH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M.","family":"SANCHEZ\u2010CASAS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. O.","family":"AYALA\u2010SULCA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"FERNANDEZ\u2010SALAS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,4,10]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268809002325"},{"key":"e_1_2_4_3_1","first-page":"115","article-title":"Polymerase chain reaction assay identifies Culex nigripalpus: part of an assay for molecular identification of the common Culex (Culex) mosquitoes of the Eastern United States.","volume":"19","author":"Aspen S.","year":"2003","journal-title":"Journal of the American Mosquito Control 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