{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:57:23Z","timestamp":1791269843743,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,4,15]],"date-time":"2004-04-15T00:00:00Z","timestamp":1081987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Important pathogens in the genus <jats:italic>Yersinia<\/jats:italic> include the plague bacillus <jats:italic>Yersinia pestis<\/jats:italic> and two enteropathogenic species, <jats:italic>Yersinia pseudotuberculosis<\/jats:italic> and <jats:italic>Yersinia enterocolitica<\/jats:italic>. A shift in growth temperature induced changes in the number and type of acyl groups on the lipid A of all three species. After growth at 37\u00b0C, <jats:italic>Y. pestis<\/jats:italic> lipopolysaccharide (LPS) contained the tetra\u2010acylated lipid IV<jats:sub>A<\/jats:sub> and smaller amounts of lipid IV<jats:sub>A<\/jats:sub> modified with C10 or C12 acyl groups, <jats:italic>Y. pseudotuberculosis<\/jats:italic> contained the same forms as part of a more heterogeneous population in which lipid IV<jats:sub>A<\/jats:sub> modified with C16:0 predominated, and <jats:italic>Y. enterocolitica<\/jats:italic> produced a unique tetra\u2010acylated lipid A. When grown at 21\u00b0C, however, the three yersiniae synthesized LPS containing predominantly hexa\u2010acylated lipid A. This more complex lipid A stimulated human monocytes to secrete tumour necrosis factor\u2010\u03b1, whereas the lipid A synthesized by the three species at 37\u00b0C did not. The <jats:italic>Y. pestis phoP<\/jats:italic> gene was required for aminoarabinose modification of lipid A, but not for the temperature\u2010dependent acylation changes. The results suggest that the production of a less immunostimulatory form of LPS upon entry into the mammalian host is a conserved pathogenesis mechanism in the genus <jats:italic>Yersinia<\/jats:italic>, and that species\u2010specific lipid A forms may be important for life cycle and pathogenicity differences.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2004.04059.x","type":"journal-article","created":{"date-parts":[[2004,5,25]],"date-time":"2004-05-25T09:45:43Z","timestamp":1085478343000},"page":"1363-1373","source":"Crossref","is-referenced-by-count":228,"title":["Variation in lipid A structure in the pathogenic yersiniae"],"prefix":"10.1111","volume":"52","author":[{"given":"Roberto","family":"Rebeil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert K.","family":"Ernst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian B.","family":"Gowen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel I.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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