{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:47:47Z","timestamp":1772160467117,"version":"3.50.1"},"reference-count":86,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,4,19]],"date-time":"2004-04-19T00:00:00Z","timestamp":1082332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Calcium signalling is little understood in filamentous fungi largely because easy and routine methods for calcium measurement in living hyphae have previously been unavailable. We have developed the recombinant aequorin method for this purpose. High levels of aequorin expression were obtained in\n                    <jats:italic>Neurospora crassa<\/jats:italic>\n                    ,\n                    <jats:italic>Aspergillus niger<\/jats:italic>\n                    and\n                    <jats:italic>Aspergillus awamori<\/jats:italic>\n                    by codon optimization of the aequorin gene. Three external stimuli (mechanical perturbation, hypo\u2010osmotic shock and high external calcium) were found transiently to increase [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>c<\/jats:sub>\n                    . Each of the calcium signatures associated with these physico\u2010chemical treatments was unique, suggesting the involvement of three distinct calcium\u2010mediated signal transduction pathways. The fungal calcium channel blocker KP4 inhibited the [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>c<\/jats:sub>\n                    responses to hypo\u2010osmotic shock and high external calcium, but not to mechanical perturbation. The divalent cation chelator BAPTA inhibited [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>c<\/jats:sub>\n                    responses to mechanical perturbation and hypo\u2010osmotic shock. The calcium agonists A23187 and cyclopiazonic acid increased [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>c<\/jats:sub>\n                    levels.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2958.2004.04066.x","type":"journal-article","created":{"date-parts":[[2004,5,25]],"date-time":"2004-05-25T05:45:43Z","timestamp":1085463943000},"page":"1437-1450","source":"Crossref","is-referenced-by-count":96,"title":["Calcium measurement in living filamentous fungi expressing codon\u2010optimized aequorin"],"prefix":"10.1111","volume":"52","author":[{"given":"G.","family":"Nelson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Kozlova\u2010Zwinderman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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