{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T14:46:06Z","timestamp":1792853166651,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,6,16]],"date-time":"2004-06-16T00:00:00Z","timestamp":1087344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Listeria monocytogenes<\/jats:italic> is a Gram\u2010positive intracellular bacterium responsible for severe opportunistic infections in humans and animals. Signature\u2010tagged mutagenesis (STM) was used to identify a gene named <jats:italic>fbpA<\/jats:italic>, required for efficient liver colonization of mice inoculated intravenously. FbpA was also shown to be required for intestinal and liver colonization after oral infection of transgenic mice expressing human E\u2010cadherin. <jats:italic>fbpA<\/jats:italic> encodes a 570\u2010amino\u2010acid polypeptide that has strong homologies to atypical fibronectin\u2010binding proteins. FbpA binds to immobilized human fibronectin in a dose\u2010dependent and saturable manner and increases adherence of wild\u2010type <jats:italic>L. monocytogenes<\/jats:italic> to HEp\u20102 cells in the presence of exogenous fibronectin. Despite the lack of conventional secretion\/anchoring signals, FbpA is detected using an antibody generated against the recombinant FbpA protein on the bacterial surface by immunofluorescence, and in the membrane compartment by Western blot analysis of cell extracts. Strikingly, FbpA expression affects the protein levels of two virulence factors, listeriolysin O (LLO) and InlB, but not that of InlA or ActA. FbpA co\u2010immunoprecipitates with LLO and InlB, but not with InlA or ActA. Thus, FbpA, in addition to being a fibronectin\u2010binding protein, behaves as a chaperone or an escort protein for two important virulence factors and appears as a novel multifunctional virulence factor of <jats:italic>L. monocytogenes<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2004.04138.x","type":"journal-article","created":{"date-parts":[[2004,7,2]],"date-time":"2004-07-02T06:32:10Z","timestamp":1088749930000},"page":"639-649","source":"Crossref","is-referenced-by-count":126,"title":["FbpA, a novel multifunctional <i>Listeria monocytogenes<\/i> virulence factor"],"prefix":"10.1111","volume":"53","author":[{"given":"S.","family":"Dramsi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Bourdichon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Cabanes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lecuit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Fsihi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Cossart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,6,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.1.78-88.1997"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02798.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.7.1972-1982.2004"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4621825.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00750.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.7.1458"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2003.03945.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-6-1615"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00146"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.10.5013"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.57.11.3629-3636.1989"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.9.3937-3946.1994"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.7.2415-2419.1996"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02297.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200202121"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00104"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07274.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90009-N"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"6698","DOI":"10.1128\/IAI.67.12.6698-6701.1999","article-title":"Listeria 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