{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T13:43:03Z","timestamp":1774791783158,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,9,28]],"date-time":"2004-09-28T00:00:00Z","timestamp":1096329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The <jats:italic>mtr<\/jats:italic> (<jats:styled-content>m<\/jats:styled-content>ultiple <jats:styled-content>t<\/jats:styled-content>ransferable <jats:styled-content>r<\/jats:styled-content>esistance) gene complex in <jats:italic>Neisseria gonorrhoeae<\/jats:italic> encodes an energy\u2010dependent efflux pump system that is responsible for export of anti\u2010bacterial hydrophobic agents. Expression of the <jats:italic>mtrCDE<\/jats:italic> operon in gonococci is negatively regulated by the MtrR protein. Hydrophobic agent resistance mediated by the <jats:italic>mtr<\/jats:italic> system is also inducible, which results from an AraC\u2010like protein termed MtrA. In this work, we identified and characterized a pump similar to the gonococcal <jats:italic>mtr<\/jats:italic> system in various strains of <jats:italic>Neisseria meningitidis<\/jats:italic>. Unlike the situation with gonococci, the <jats:italic>mtr<\/jats:italic> system in meningococci is not subject to the MtrR or MtrA regulatory schemes. An analysis of the promoter region of the <jats:italic>mtrCDE<\/jats:italic> operon in a panel of meningococcal strains revealed the presence of one or two classes of insertion sequence elements. A 155\u2013159\u2003bp insertion sequence element known as the Correia element, previously identified elsewhere in the gonococcal and meningococcal genomes, was present in the <jats:italic>mtrCDE<\/jats:italic> promoter region of all meningococcal strains tested. In addition to the Correia element, a minority of strains had a tandemly linked, intact copy of IS<jats:italic>1301<\/jats:italic>. As described previously, a binding site for the integration host factor (IHF) was present at the centre of the Correia element upstream of <jats:italic>mtrCDE<\/jats:italic> genes. IHF was found to bind specifically to this site and deletion of the IHF binding site enhanced <jats:italic>mtrC<\/jats:italic> transcription. We also identified a post\u2010transcriptional regulation of the <jats:italic>mtrCDE<\/jats:italic> transcript by cleavage in the inverted repeat of the Correia element, as previously described by <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#b25\">Mazzone <jats:italic>et\u2003al<\/jats:italic>. [<jats:italic>Gene<\/jats:italic><jats:bold>278<\/jats:bold>: 211\u2013222 (2001)]<\/jats:ext-link> and <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#b27\">De Gregorio <jats:italic>et\u2003al<\/jats:italic>. [<jats:italic>Biochim Biophys Acta<\/jats:italic> 1576: 39\u201344 (2002)]<\/jats:ext-link>for other Correia element. We conclude that the <jats:italic>mtr<\/jats:italic> efflux system in meningococci is subject to transcriptional regulation by IHF and post\u2010transcriptional regulation by cleavage in the inverted repeat of the Correia element.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2004.04299.x","type":"journal-article","created":{"date-parts":[[2004,10,15]],"date-time":"2004-10-15T16:02:17Z","timestamp":1097856137000},"page":"731-741","source":"Crossref","is-referenced-by-count":67,"title":["Modulation of the <i>mtrCDE<\/i>\u2010encoded efflux pump gene complex of <i>Neisseria meningitidis<\/i> due to a Correia element insertion sequence"],"prefix":"10.1111","volume":"54","author":[{"given":"Corinne E.","family":"Rouquette\u2010Loughlin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline T.","family":"Balthazar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart 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