{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T01:28:42Z","timestamp":1773192522551,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,10,6]],"date-time":"2004-10-06T00:00:00Z","timestamp":1097020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Borrelia burgdorferi<\/jats:italic>, the aetiologic agent of Lyme disease, modulates gene expression in response to changes imposed by its arthropod vector and mammalian hosts. As reactive oxygen species (ROS) are known to vary in these environments, we asked how <jats:italic>B. burgdorferi<\/jats:italic> responds to oxidative stress. The <jats:italic>B. burgdorferi<\/jats:italic> genome encodes a PerR homologue (recently designated BosR) that represses the oxidative stress response in other bacteria, suggesting a similar function in <jats:italic>B. burgdorferi<\/jats:italic>. When we tested the sensitivity of <jats:italic>B. burgdorferi<\/jats:italic> to ROS, one clonal non\u2010infectious <jats:italic>B. burgdorferi<\/jats:italic> isolate exhibited hypersensitivity to <jats:italic>t<\/jats:italic>\u2010butyl hydroperoxide when compared with infectious <jats:italic>B. burgdorferi<\/jats:italic> and other non\u2010infectious isolates. Sequence analysis indicated that the hypersensitive non\u2010infectious isolates <jats:italic>bosR<\/jats:italic> allele contained a single nucleotide substitution, converting an arginine to a lysine (<jats:italic>bosRR39K<\/jats:italic>). Mutants in <jats:italic>bosRR39K<\/jats:italic> exhibited an increase in resistance to oxidative stressors when compared with the parental non\u2010infectious strain, suggesting that BosRR39K functioned as a repressor. Complementation with <jats:italic>bosRR39K<\/jats:italic> and <jats:italic>bosR<\/jats:italic> resulted in differential sensitivity to <jats:italic>t<\/jats:italic>\u2010butyl hydroperoxide, indicating that these alleles are functionally distinct. In contrast to BosR, BosRR39K did not activate transcription of a <jats:italic>napA<\/jats:italic> promoter\u2013<jats:italic>lacZ<\/jats:italic> reporter in <jats:italic>Escherichia coli<\/jats:italic> nor bind the <jats:italic>napA<\/jats:italic> promoter\/operator domain. However, we found that both BosR and BosRR39K bound to the putative promoter\/operator region of superoxide dismutase (<jats:italic>sodA<\/jats:italic>). In addition, we determined that cells lacking BosRR39K\u200a synthesized\u200a fourfold\u200a greater\u200a levels\u200a of the decorin binding adhesin DbpA suggesting that BosRR39K regulates genes unrelated to oxidative stress. Based on these data, we propose that the single amino acid substitution, R39K, dramatically alters the activity of BosR by altering its ability to bind DNA at target regulatory sequences.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2004.04352.x","type":"journal-article","created":{"date-parts":[[2004,11,19]],"date-time":"2004-11-19T09:57:40Z","timestamp":1100858260000},"page":"1352-1363","source":"Crossref","is-referenced-by-count":57,"title":["A conservative amino acid change alters the function of BosR, the redox regulator of <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1111","volume":"54","author":[{"given":"J.","family":"Seshu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie A.","family":"Boylan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny A.","family":"Hyde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristen L.","family":"Swingle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank C.","family":"Gherardini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan T.","family":"Skare","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2325"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80312-8"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(71)90370-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.9.2445-2452.2000"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2032956100"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3371-3383.2003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.7.3181-3187.1999"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.18.8190"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00300-2"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119264"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.20.6223-6229.1999"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.22.6723-6727.2003"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306710200"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.12.3276-3286.2002"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306845101"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01103.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02543.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231442498"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.446-455.2001"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4608-4613.2003"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)00849-5"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3670-3677.2001"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03015.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.2.670"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1689-1705.2003"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200419894"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03337.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5471.1651"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.25.13865"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03452.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032667699"},{"key":"e_1_2_6_34_1","first-page":"253","article-title":"Electrotransformation of the spirochete Borrelia burgdorferi","volume":"47","author":"Samuels D.S.","year":"1995","journal-title":"Methods Mol Biol"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.7.2909"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.3.1580-1586.2004"},{"key":"e_1_2_6_37_1","doi-asserted-by":"crossref","first-page":"4407","DOI":"10.1128\/IAI.67.9.4407-4417.1999","article-title":"Cloning and molecular characterization of plasmid\u2010encoded antigens of Borrelia burgdorferi","volume":"67","author":"Skare J.T.","year":"1999","journal-title":"Infect Immun"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(99)80033-2"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02104.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1834315100"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5357.1718"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.15.4639-4643.1999"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.15.4571-4579.2001"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2958.2004.04352.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2958.2004.04352.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T10:00:47Z","timestamp":1697277647000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2958.2004.04352.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10,6]]},"references-count":42,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1111\/j.1365-2958.2004.04352.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2958.2004.04352.x","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,10,6]]}}}