{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:58:48Z","timestamp":1790985528742,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,12,2]],"date-time":"2004-12-02T00:00:00Z","timestamp":1101945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    In\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    the promoter region of the\n                    <jats:italic>svpA\u2010srtB<\/jats:italic>\n                    locus contains a well\u2010conserved Fur box. We characterized the iron\u2010regulation of this locus: real\u2010time polymerase chain reaction analyses and anti\u2010SvpA immunoblots showed that, in response to iron deprivation\n                    <jats:italic>svpA<\/jats:italic>\n                    transcription and SvpA production markedly increased (80\u2010fold and 10\u2010fold respectively), when initiated by either the addition of the iron chelator 2,2\u2032\u2010bipyridyl to BHI media, or by growth in iron\u2010restricted minimal media. Green fluorescent protein (GFP) reporter constructs also showed increased activity of the\n                    <jats:italic>svpA\u2010srtB<\/jats:italic>\n                    promoter in\n                    <jats:italic>Escherichia coli (<\/jats:italic>\n                    37\u2010fold) and in\n                    <jats:italic>L.\u2003monocytogenes<\/jats:italic>\n                    (two\u2010 to threefold) when the bacteria were grown in iron\u2010deficient conditions. A \u0394\n                    <jats:italic>fur<\/jats:italic>\n                    mutant of\n                    <jats:italic>L.\u2003monocytogenes<\/jats:italic>\n                    constitutively synthesized SvpA, as well as GFP fused to the\n                    <jats:italic>svpA\u2010srtB<\/jats:italic>\n                    promoter. Cellular fractionation data revealed that in iron\u2010rich media wild\u2010type SvpA was exclusively secreted to the culture supernatant. However, both the \u0394\n                    <jats:italic>fur<\/jats:italic>\n                    derivative and wild\u2010type\n                    <jats:italic>L.\u2003monocytogenes<\/jats:italic>\n                    grown in iron\u2010deficient media anchored a fraction of the SvpA proteins (\u223c5%) to peptidoglycan, and produced a lower\u2010molecular weight, wholly secreted form of SvpA. Together these data establish that iron availability controls transcription of the\n                    <jats:italic>svpA\u2010srtB<\/jats:italic>\n                    locus (through Fur\u2010mediated regulation), and attachment of SvpA to the cell wall (through SrtB\u2010mediated covalent linkage). SvpA bears homology to IsdC, a haemin\u2010binding protein of\n                    <jats:italic>Staphylococcus aureus,<\/jats:italic>\n                    and haemin bound to SvpA in solution. However, site\u2010directed deletions of four structural genes and the promoter of the\n                    <jats:italic>svpA\u2010srtB<\/jats:italic>\n                    locus did not impair haemin, haemoglobin or ferrichrome utilization in nutrition tests. We did not find strong evidence to support the notion that the\n                    <jats:italic>svpA\u2010srtB<\/jats:italic>\n                    locus participates in haemin acquisition, as was reported for the homologous\n                    <jats:italic>isd<\/jats:italic>\n                    operon of\n                    <jats:italic>S.\u2003aureus<\/jats:italic>\n                    . Furthermore, the\n                    <jats:italic>svpA\u2010srtB<\/jats:italic>\n                    mutant strains showed no significant attenuation of virulence in an intravenous mouse model system, but we found that the mutations reduced the persistence of\n                    <jats:italic>L.\u2003monocytogenes<\/jats:italic>\n                    in murine liver, spleen and intestines after oral administration.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2958.2004.04436.x","type":"journal-article","created":{"date-parts":[[2005,1,30]],"date-time":"2005-01-30T12:21:29Z","timestamp":1107087689000},"page":"927-940","source":"Crossref","is-referenced-by-count":59,"title":["The\n                    <i>svpA\u2010srtB<\/i>\n                    locus of\n                    <i>Listeria monocytogenes:<\/i>\n                    Fur\u2010mediated iron regulation and effect on virulence"],"prefix":"10.1111","volume":"55","author":[{"given":"Salete M. 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