{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T01:54:42Z","timestamp":1793930082635,"version":"4.8.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,1,17]],"date-time":"2005-01-17T00:00:00Z","timestamp":1105920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The infectious agent of Legionnaires\u2019 disease,\n                    <jats:italic>Legionella (L) pneumophila<\/jats:italic>\n                    , multiplies intracellularly in eukaryotic cells. This study has been performed to explore the nutrient requirements of\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    during intracellular replication. In human monocytes, bacterial replication rate was reduced by 76% in defined medium lacking\n                    <jats:sc>l<\/jats:sc>\n                    \u2010cysteine,\n                    <jats:sc>l<\/jats:sc>\n                    \u2010glutamine or\n                    <jats:sc>l<\/jats:sc>\n                    \u2010serine. SLC1A5 (hATB\n                    <jats:sup>0,+<\/jats:sup>\n                    ), a neutral amino acid transporter, was upregulated in the host cells after infection with\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    . Inhibition of SLC1A5 by BCH, a competitive inhibitor of amino acid uptake as well as siRNA silencing of the\n                    <jats:italic>slc1a5<\/jats:italic>\n                    gene blocked intracellular multiplication of\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    without compromising viability of host cells. These observations suggest that replication of\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    depends on the function of host cell SLC1A5.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2958.2005.04490.x","type":"journal-article","created":{"date-parts":[[2005,2,17]],"date-time":"2005-02-17T10:17:07Z","timestamp":1108635427000},"page":"1528-1537","source":"Crossref","is-referenced-by-count":83,"title":["Intracellular multiplication of\n                    <i>Legionella pneumophila<\/i>\n                    depends on host cell amino acid transporter 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