{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:54:10Z","timestamp":1791129250412,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,18]],"date-time":"2005-02-18T00:00:00Z","timestamp":1108684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Sinorhizobium meliloti<\/jats:italic>, a legume symbiont and <jats:italic>Brucella abortus<\/jats:italic>, a phylogenetically related mammalian pathogen, both require their BacA proteins to establish chronic intracellular infections in their respective hosts. The lipid A molecules of <jats:italic>S. meliloti<\/jats:italic> and <jats:italic>B. abortus<\/jats:italic> are unusually modified with a very\u2010long\u2010chain fatty acid (VLCFA; C\u2003\u2265\u200328) and we discovered that BacA is involved in this unusual modification. This observation raised the possibility that the unusual lipid A modification could be crucial for the chronic infection of both <jats:italic>S. meliloti<\/jats:italic> and <jats:italic>B. abortus<\/jats:italic>. We investigated this by constructing and characterizing <jats:italic>S. meliloti<\/jats:italic> mutants in the <jats:italic>lpxXL<\/jats:italic> and <jats:italic>acpXL<\/jats:italic> genes, which encode an acyl transferase and acyl carrier protein directly involved in the biosynthesis of VLCFA\u2010modified lipid A. Our analysis revealed that the unusually modified lipid A is important, but not crucial, for <jats:italic>S. meliloti<\/jats:italic> chronic infection and that BacA must have an additional function, which in combination with its observed effect on the lipid A in the free\u2010living form of <jats:italic>S. meliloti<\/jats:italic>, is essential for the chronic infection. Additionally, we discovered that in the absence of VLCFAs, <jats:italic>S. meliloti<\/jats:italic> produces novel pentaacylated lipid A species, modified with unhydroxylated fatty acids, which are important for stress resistance.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2005.04536.x","type":"journal-article","created":{"date-parts":[[2005,3,9]],"date-time":"2005-03-09T12:41:36Z","timestamp":1110372096000},"page":"68-80","source":"Crossref","is-referenced-by-count":81,"title":["Importance of unusually modified lipid A in <i>Sinorhizobium<\/i> stress resistance and legume symbiosis"],"prefix":"10.1111","volume":"56","author":[{"given":"Gail P.","family":"Ferguson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anup","family":"Datta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russ 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