{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:52:52Z","timestamp":1791165172924,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,18]],"date-time":"2005-02-18T00:00:00Z","timestamp":1108684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Listeria monocytogenes<\/jats:italic> is a pathogen that causes listeriosis, a severe food\u2010borne infection. This bacterium, in order to survive and grow in the multiple conditions encountered in the host and the environment, has evolved a large number of regulatory elements, in particular many signal transduction systems based on reversible phosphorylation. The genome sequence has revealed genes for 16 putative two\u2010component systems, four putative tyrosine phosphatases, three putative serine\u2010threonine kinases and two putative serine\u2010threonine phosphatases. We found that one of the latter genes, <jats:italic>stp<\/jats:italic>, encodes a functional Mn<jats:sup>2+<\/jats:sup>\u2010dependent serine\u2010threonine phosphatase similar to PPM eukaryotic phosphatases (Mg<jats:sup>2+<\/jats:sup>\u2010or Mn<jats:sup>2+<\/jats:sup>\u2010dependent protein phosphatase) and is required for growth of <jats:italic>L. monocytogenes<\/jats:italic> in a murine model of infection. We identified as the first target for Stp, the elongation factor EF\u2010Tu. Post\u2010translational phosphorylation of EF\u2010Tu had been shown to prevent its binding to amino\u2010acylated transfer RNA as well as to kirromycin, an antibiotic known to inhibit EF\u2010Tu function. Accordingly, an <jats:italic>stp<\/jats:italic> deletion mutant is less sensitive to kirromycin. These results suggest an important role for Stp in regulating EF\u2010Tu and controlling bacterial survival in the infected host.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2005.04551.x","type":"journal-article","created":{"date-parts":[[2005,3,24]],"date-time":"2005-03-24T10:35:50Z","timestamp":1111660550000},"page":"383-396","source":"Crossref","is-referenced-by-count":106,"title":["Translation elongation factor EF\u2010Tu is a target for Stp, a serine\u2010threonine phosphatase involved in virulence of <i>Listeria monocytogenes<\/i>"],"prefix":"10.1111","volume":"56","author":[{"given":"Cristel","family":"Archambaud","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith","family":"Gouin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Pizarro\u2010Cerda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Cossart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Dussurget","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,3,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.24.14541"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601629"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.27.1.133"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560050720"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2002.03290.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"2112","DOI":"10.1128\/AEM.65.5.2112-2115.1999","article-title":"Mutational analysis of the role of HPr in Listeria monocytogenes","volume":"65","author":"Christensen D.P.","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.11.3355-3362.2004"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04085.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-003-0640-6"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03207.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb01071.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04138.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.57.030502.090934"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2434-2437.2004"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.22.6109-6114.2002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/361730a0"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2160-2169.2004"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.8.7768349"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)30005-5"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/261023a0"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01652.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb00928.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3404-3412.2002"},{"issue":"3","key":"e_1_2_7_25_1","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1242\/jcs.105.3.699","article-title":"Polarized distribution of Listeria monocytogenes surface protein ActA at the site of directional actin assembly","volume":"105","author":"Kocks C.","year":"1993","journal-title":"J Cell Sci"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02816232"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02566.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200400997"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.3.947-952.1992"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.8.10.1038"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54193-4"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03178.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02289.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1128\/IAI.66.6.2625-2631.1998","article-title":"Mapping and identification of the major cell wall\u2010associated components of Mycobacterium leprae","volume":"66","author":"Marques M.A.","year":"1998","journal-title":"Infect Immun"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02816231"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03413.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010824200"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209210200"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03572.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02879.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.19.5634-5638.2000"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.39.100185.003013"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(96)01205-6"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0580-9517(02)31009-2"},{"key":"e_1_2_7_45_1","volume-title":"Control of the Elongation Phase of Protein Synthesis.","author":"Proud C.G.","year":"2000"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M212747200"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.43.30337"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00382.x"},{"key":"e_1_2_7_49_1","volume-title":"Molecular Cloning: A Laboratory Manual.","author":"Sambrook J.E.","year":"1989"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.04.164"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.0217502"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1998.tb00369.x"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(84)90161-6"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02362.x"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-002-1045-1"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.584-640.2001"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099384"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02483.x"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2958.2005.04551.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2958.2005.04551.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T10:35:04Z","timestamp":1695897304000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2958.2005.04551.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3,7]]},"references-count":57,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,4]]}},"alternative-id":["10.1111\/j.1365-2958.2005.04551.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2958.2005.04551.x","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3,7]]}}}