{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T09:51:49Z","timestamp":1785232309017,"version":"3.55.0"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,8,26]],"date-time":"2005-08-26T00:00:00Z","timestamp":1125014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The human gastric pathogenic bacterium <jats:italic>Helicobacter pylori<\/jats:italic> lacks a MutSLH\u2010like DNA mismatch repair system. Here, we have investigated the functional roles of a <jats:italic>mutS<\/jats:italic> homologue found in <jats:italic>H. pylori<\/jats:italic>, and show that it plays an important physiological role in repairing oxidative DNA damage. <jats:italic>H. pylori mutS<\/jats:italic> mutants are more sensitive than wild\u2010type cells to oxidative stress induced by agents such as H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, paraquat or oxygen. Exposure of <jats:italic>mutS<\/jats:italic> cells to oxidative stress results in a significant (\u223c10\u2010fold) elevation of mutagenesis. Strikingly, most mutations in <jats:italic>mutS<\/jats:italic> cells under oxidative stress condition are G:C to T:A transversions, a signature of 8\u2010oxoguanine (8\u2010oxoG). Purified <jats:italic>H. pylori<\/jats:italic> MutS protein binds with a high specific affinity to double\u2010stranded DNA (dsDNA) containing 8\u2010oxoG as well as to DNA Holliday junction structures, but only weakly to dsDNA containing a G:A mismatch. Under oxidative stress conditions, <jats:italic>mutS<\/jats:italic> cells accumulate higher levels (approximately threefold) of 8\u2010oxoG DNA lesions than wild\u2010type cells. Finally, we observe that <jats:italic>mutS<\/jats:italic> mutant cells have reduced colonization capacity in comparison to wild\u2010type cells in a mouse infection model.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2005.04833.x","type":"journal-article","created":{"date-parts":[[2005,8,26]],"date-time":"2005-08-26T09:07:52Z","timestamp":1125047272000},"page":"166-176","source":"Crossref","is-referenced-by-count":72,"title":["The <i>Helicobacter pylori<\/i> MutS protein confers protection from oxidative DNA damage"],"prefix":"10.1111","volume":"58","author":[{"given":"Ge","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen","family":"Alamuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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