{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:23:22Z","timestamp":1792185802058,"version":"4.4.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,9,22]],"date-time":"2005-09-22T00:00:00Z","timestamp":1127347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Macrophage infectivity potentiators (MIPs) are a family of surface\u2010exposed virulence factors of intracellular microorganisms such as <jats:italic>Legionella<\/jats:italic>, <jats:italic>Chlamydia<\/jats:italic> and <jats:italic>Trypanosoma<\/jats:italic>. These proteins display peptidyl\u2010prolyl <jats:italic>cis<\/jats:italic>\/<jats:italic>trans<\/jats:italic> isomerase (PPIase) activity that is inhibited by immunosuppressants FK506 and rapamycin. Here we describe the identification and characterization in <jats:italic>Neisseria gonorrhoeae<\/jats:italic> of Ng\u2010MIP, a surface\u2010exposed lipoprotein with high homology to MIPs. The protein is an homodimer with rapamycin\u2010inhibited PPIase activity confirming that it is a functional member of the MIP family. A knock\u2010out strain, generated by deletion of the <jats:italic>mip<\/jats:italic> gene in <jats:italic>N. gonorrhoeae<\/jats:italic> F62 strain, was evaluated for its role in infection of mouse and human macrophages. We show that Ng\u2010MIP promotes the intracellular survival of <jats:italic>N. gonorrhoeae<\/jats:italic> in macrophages, highlighting a possible role of this protein in promoting the persistence of gonococcal infection.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2005.04859.x","type":"journal-article","created":{"date-parts":[[2005,9,22]],"date-time":"2005-09-22T19:19:52Z","timestamp":1127416792000},"page":"669-681","source":"Crossref","is-referenced-by-count":110,"title":["Ng\u2010MIP, a surface\u2010exposed lipoprotein of <i>Neisseria gonorrhoeae<\/i>, has a peptidyl\u2010prolyl <i>cis<\/i>\/<i>trans<\/i> isomerase (PPIase) activity and is involved in persistence in macrophages"],"prefix":"10.1111","volume":"58","author":[{"given":"Rosanna","family":"Leuzzi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Serino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Scarselli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Savino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rita","family":"Fontana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Monaci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annarita","family":"Taddei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunter","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rino","family":"Rappuoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariagrazia","family":"Pizza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,9,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.2.507"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.11.5188"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1128\/iai.57.4.1255-1262.1989","article-title":"A Legionella pneumophila gene encoding a species\u2010specific surface protein potentiates initiation of intracellular infection","volume":"57","author":"Cianciotto N.P.","year":"1989","journal-title":"Infect Immun"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.1.121"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00293641"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00296200"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb00858.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1997.tb00311.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb00917.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.2.1016-1019.2003"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.65.10.4122-4129.1997"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4389-4397.2003"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.13.3441-3447.1998"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb01831.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01168.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/5.Supplement_4.S708"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-141-11-2861"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07245.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvf009"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0901-779"},{"key":"e_1_2_6_22_1","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.4.1164"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00970-8"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00951-5"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1702904"},{"key":"e_1_2_6_27_1","first-page":"1965","article-title":"Monoclonal antibodies to gonococcal pili: studies on antigenic determinants on pili from variants of strain P9","volume":"129","author":"Virji M.","year":"1983","journal-title":"J Gen Microbiol"},{"key":"e_1_2_6_28_1","first-page":"593","volume-title":"Sexually Transmitted Diseases.","author":"Westrom L.","year":"1990"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.12.4576-4583.1995"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2958.2005.04859.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2958.2005.04859.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T13:31:56Z","timestamp":1695821516000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2958.2005.04859.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9,22]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,11]]}},"alternative-id":["10.1111\/j.1365-2958.2005.04859.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2958.2005.04859.x","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,9,22]]}}}