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This permease contains 480 amino acids with 11 predicted <jats:italic>trans\u2010<\/jats:italic>membrane domains. Expressing <jats:italic>LdAAP3<\/jats:italic> in <jats:italic>Saccharomyces cerevisiae<\/jats:italic> mutants revealed that <jats:italic>LdAAP3<\/jats:italic> codes for a high\u2010affinity arginine transporter (<jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> 1.9\u2003\u00b5M). LdAAP3 is highly specific for arginine as its transport was not inhibited by other amino acids or arginine\u2010related compounds. Using green fluorescence protein (GFP) fused to the N\u2010terminus of LdAAP3, this transporter was localized to the surface membrane of promastigotes. The GFP\u2013LdAAP3 chimera mediated a threefold increase in arginine transport in promastigotes, indicating that it is active and confirmed that <jats:italic>LdAAP3<\/jats:italic> codes for an arginine transporter in parasite cells as well. LdAAP3 is novel as it shares a high level of homology with amino acid permeases from other trypanosomatidae but almost none with permeases from other phyla. The results of this work suggest that LdAAP3 might play a role in host\u2013parasite interaction.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2006.05060.x","type":"journal-article","created":{"date-parts":[[2006,2,21]],"date-time":"2006-02-21T11:06:10Z","timestamp":1140519970000},"page":"30-38","source":"Crossref","is-referenced-by-count":82,"title":["A novel high\u2010affinity arginine transporter from the human parasitic protozoan <i>Leishmania donovani<\/i>"],"prefix":"10.1111","volume":"60","author":[{"given":"Pninit","family":"Shaked\u2010Mishan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Suter\u2010Grotemeyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamar","family":"Yoel\u2010Almagor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neta","family":"Holland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zilberstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Rentsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,2,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.09.212"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2005.02.004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/340820"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1768-322X.1996.tb00962.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.07.002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0630165100"},{"key":"e_1_2_6_8_1","first-page":"21","article-title":"The 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