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We recently have demonstrated that despite the high degree of similarity between the promoters of the <jats:italic>ospF<\/jats:italic> (P<jats:sub><jats:italic>ospF<\/jats:italic><\/jats:sub>) and <jats:italic>ospE<\/jats:italic> (P<jats:sub><jats:italic>ospE<\/jats:italic><\/jats:sub>) genes of <jats:italic>B.\u2003burgdorferi<\/jats:italic> strain 297, the differential expression of <jats:italic>ospF<\/jats:italic> is RpoS\u2010dependent, while <jats:italic>ospE<\/jats:italic> is controlled by \u03c3<jats:sup>70<\/jats:sup>. Herein we used wild\u2010type and RpoS\u2010deficient strains of <jats:italic>B.\u2003burgdorferi<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic> to analyse transcriptional reporters consisting of a green fluorescent protein (<jats:italic>gfp<\/jats:italic>) gene fused to P<jats:sub><jats:italic>ospF<\/jats:italic><\/jats:sub>, P<jats:sub><jats:italic>ospE<\/jats:italic><\/jats:sub>, or two hybrid promoters in which the \u221210 regions of P<jats:sub><jats:italic>ospF<\/jats:italic><\/jats:sub> and P<jats:sub><jats:italic>ospE<\/jats:italic><\/jats:sub> were switched [P<jats:italic><jats:sub>ospF <\/jats:sub><\/jats:italic><jats:sup>(E\u2003\u2212\u200310)<\/jats:sup> and P<jats:italic><jats:sub>ospE<\/jats:sub><\/jats:italic>\u200a<jats:sup>(F\u2003\u2212\u200310)<\/jats:sup> respectively]. We found that the P<jats:sub><jats:italic>ospF<\/jats:italic><\/jats:sub>\u221210 region is both necessary and sufficient for RpoS\u2010dependent recognition in <jats:italic>B.\u2003burgdorferi<\/jats:italic>, while \u03c3<jats:sup>70<\/jats:sup> specificity for P<jats:sub><jats:italic>ospE<\/jats:italic><\/jats:sub> is dependent on elements outside of the \u221210 region. In <jats:italic>E.\u2003coli<\/jats:italic>, sigma factor selectivity for these promoters was much more permissive, with expression of each being primarily due to \u03c3<jats:sup>70<\/jats:sup>. Alignment of the sequences upstream of each of the <jats:italic>ospE\/ospF\/elp<\/jats:italic> genes from <jats:italic>B.\u2003burgdorferi<\/jats:italic> strains 297 and B31 revealed that two B31 <jats:italic>ospF<\/jats:italic> paralogues [<jats:italic>erpK<\/jats:italic> (BBM38) and <jats:italic>erpL<\/jats:italic> (BBO39)] have \u221210 regions virtually identical to that of P<jats:sub><jats:italic>ospF<\/jats:italic><\/jats:sub>. Correspondingly, expression of <jats:italic>gfp<\/jats:italic> reporters based on the <jats:italic>erpK<\/jats:italic> and <jats:italic>erpL<\/jats:italic> promoters was RpoS\u2010dependent. Thus, the sequence of the P<jats:sub><jats:italic>ospF<\/jats:italic><\/jats:sub>\u221210 region appears to serve as a motif for RpoS recognition, the first described for any <jats:italic>B.\u2003burgdorferi<\/jats:italic> promoter. Taken together, our data support the notion that <jats:italic>B.\u2003burgdorferi<\/jats:italic> utilizes sequence differences at the \u221210 region as one mechanism for maintaining the transcriptional integrity of RpoS\u2010dependent and \u2010independent genes activated at the onset of tick feeding.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2006.05066.x","type":"journal-article","created":{"date-parts":[[2006,2,8]],"date-time":"2006-02-08T14:33:40Z","timestamp":1139409220000},"page":"1859-1875","source":"Crossref","is-referenced-by-count":42,"title":["Sigma factor selectivity in <i>Borrelia burgdorferi<\/i>: RpoS recognition of the <i>ospE\/ospF\/elp<\/i> promoters is dependent on the sequence of the \u221210 region"],"prefix":"10.1111","volume":"59","author":[{"given":"Christian H.","family":"Eggers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa 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