{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:07:46Z","timestamp":1760954866972},"reference-count":66,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>When <jats:italic>Borrelia burgdorferi<\/jats:italic> (Bb) is transmitted from a tick vector to a mammalian host the spirochaete alters gene expression, allowing for adaptation to the new host. We evaluated the regulation of paralogous gene family (pgf) 54 members in response to environmental cues and focused our efforts on determining the molecular mechanisms influencing <jats:italic>bba66<\/jats:italic> expression. By qRT\u2010PCR, <jats:italic>bba65<\/jats:italic>, <jats:italic>bba66<\/jats:italic>, <jats:italic>bba71<\/jats:italic> and <jats:italic>bba73<\/jats:italic> displayed regulation similar to <jats:italic>ospC<\/jats:italic> under mammalian\u2010like conditions. Of the pgf 54 members, <jats:italic>bba66<\/jats:italic> demonstrated the greatest and second greatest change in expression in response to pH or temperature shift respectively. Furthermore, Bb\u2010infected mice and patients with early disseminated Lyme disease produced detectable antibodies to BBA66. A protein(s) active in Bb at pH\u20037 was able to interact with the <jats:italic>bba66<\/jats:italic> upstream region and was specific as <jats:italic>bba64<\/jats:italic> and <jats:italic>ospC<\/jats:italic> promoters were unable to out\u2010compete for binding. <jats:italic>bba66<\/jats:italic> promoter mapping revealed putative \u03c3<jats:sup>70<\/jats:sup> and \u03c3<jats:sup>S<\/jats:sup> consensus sequences, enabling us to narrow the protein binding site to a region within an imperfect inverted repeat upstream of the \u221235 region. Moreover, BBA66 production is associated with an infectious phenotype, and loss of either \u03c3<jats:sup>N<\/jats:sup> or \u03c3<jats:sup>S<\/jats:sup> resulted in loss of BBA66. Promoter\u2010GFP fusion analysis indicated that the \u03c3<jats:sup>70<\/jats:sup> and\/or \u03c3<jats:sup>S<\/jats:sup> consensus sequences alone were not sufficient to initiate transcription and a portion of the upstream inverted repeat was required. These results suggest a primary role for BBA66 in Bb transmission and infection.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2006.05224.x","type":"journal-article","created":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T09:35:10Z","timestamp":1149154510000},"page":"243-258","source":"Crossref","is-referenced-by-count":27,"title":["Regulation and expression of <i>bba66<\/i> encoding an immunogenic infection\u2010associated lipoprotein in <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1111","volume":"61","author":[{"given":"Dawn R.","family":"Clifton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christi L.","family":"Nolder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica L.","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"Nowalk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1988.tb31864.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1222-1230.2000"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.4146-4153.2001"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.9.2745-2756.2004"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.26.3.475-478.1988"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.41.2.795-804.1983"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.9.2445-2452.2000"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3371-3383.2003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7043737"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.1991.tb03056.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.2.392-398.1996"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.7.3181-3187.1999"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6677-6684.2000"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5286-5293.2001"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26165-0"},{"key":"e_1_2_6_17_1","doi-asserted-by":"crossref","first-page":"5379","DOI":"10.1128\/IAI.66.11.5379-5387.1998","article-title":"DbpA, but not OspA, is expressed by Borrelia burgdorferi during spirochetemia and is a target for protective antibodies","volume":"66","author":"Cassatt D.R.","year":"1998","journal-title":"Infect Immun"},{"key":"e_1_2_6_18_1","first-page":"365","article-title":"Lyme disease \u2013 United States, 2001\u201302","volume":"53","author":"Centers for Disease Control and Prevention","year":"2004","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1107\/S090744490400472X"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3419-3428.2003"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5063-5072.2004"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.21.7390-7402.2004"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05066.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.2139-2150.2002"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000073406"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.16.3667"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02573.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408536102"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_2_6_30_1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/jcm.35.1.86-91.1997","article-title":"Molecular characterization of a 35\u2010kilodalton protein of Borrelia burgdorferi, an antigen of diagnostic importance in early Lyme disease","volume":"35","author":"Gilmore R.D.","year":"1997","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01435-6"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3618-3627.2001"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231442498"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.2.522-525.2000"},{"key":"e_1_2_6_35_1","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1128\/iai.65.4.1165-1171.1997","article-title":"Cell\u2010density\u2010dependent expression of Borrelia burgdorferi lipoproteins in vitro","volume":"65","author":"Indest K.J.","year":"1997","journal-title":"Infect Immun"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200106)31:6<1674::AID-IMMU1674>3.0.CO;2-2"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7800-7809.2001"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308343200"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7398-7405.2005"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020770"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)90613-0"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1994.tb07242.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/160.2.351"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.60.11.4662-4672.1992"},{"key":"e_1_2_6_46_1","doi-asserted-by":"crossref","unstructured":"Nowalk A.J. Gilmore R.D. JrandCarroll J.A.(2006)Serologic proteome analysis ofBorrelia burgdorferimembrane\u2010associated proteins.Infect Immun74(in press).","DOI":"10.1128\/IAI.00189-06"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(02)58088-5"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1689-1705.2003"},{"key":"e_1_2_6_49_1","doi-asserted-by":"crossref","first-page":"5097","DOI":"10.1128\/iai.61.12.5097-5105.1993","article-title":"Molecular characterization and expression of p23 (OspC) from a North American strain of Borrelia burgdorferi","volume":"61","author":"Padula S.J.","year":"1993","journal-title":"Infect Immun"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2739-2742.2001"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032667699"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/7.1.41"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/156.5.852-a"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.56.8.1831-1836.1988"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.7.2909"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28317-0"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198303313081301"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.11.4535-4539.1995"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02256.x"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5419-5432.2004"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1998.tb00364.x"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.6008-6018.1999"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02104.x"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.4159-4163.2001"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1834315100"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.14.4822-4829.2005"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2958.2006.05224.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2958.2006.05224.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T11:13:20Z","timestamp":1697022800000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2958.2006.05224.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6]]},"references-count":66,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,7]]}},"alternative-id":["10.1111\/j.1365-2958.2006.05224.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2958.2006.05224.x","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"type":"print","value":"0950-382X"},{"type":"electronic","value":"1365-2958"}],"subject":[],"published":{"date-parts":[[2006,6]]}}}