{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:17:53Z","timestamp":1791267473470,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,6,27]],"date-time":"2006-06-27T00:00:00Z","timestamp":1151366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Many bacterial pathogens require a functional type IV secretion system (T4SS) for virulence. <jats:italic>Legionella pneumophila<\/jats:italic>, the causative agent of Legionnaires' disease, employs the Dot\/Icm T4SS to inject a large number of protein substrates into its host, thereby altering phagosome trafficking. The <jats:italic>L.\u2003pneumophila<\/jats:italic> T4SS substrate SdeA has been shown to require the accessory factor IcmS for its export. IcmS, defined as a type IV adaptor, exists as a heterodimer with IcmW and this complex functions in a manner similar to a type III secretion chaperone. Here we report an interaction between IcmS and the previously identified virulence factor LvgA. Similar to the <jats:italic>icmS<\/jats:italic> mutant, the <jats:italic>lvgA<\/jats:italic> mutant appears to assemble a fully functional Dot\/Icm complex. Both LvgA and IcmS are small, acidic proteins localized to the cytoplasm and are not exported by the Dot\/Icm system, suggesting they form a novel type IV adaptor complex. Inactivation of <jats:italic>lvgA<\/jats:italic> causes a minimal defect in growth in the human monocytic cell line U937 and the environmental host <jats:italic>Acanthamoeba castellanii<\/jats:italic>. However, the <jats:italic>lvgA<\/jats:italic> mutant was severely attenuated for intracellular growth of <jats:italic>L.\u2003pneumophila<\/jats:italic> in mouse macrophages, suggesting LvgA may be a critical factor that confers host specificity.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2006.05243.x","type":"journal-article","created":{"date-parts":[[2006,6,27]],"date-time":"2006-06-27T14:28:48Z","timestamp":1151418528000},"page":"596-613","source":"Crossref","is-referenced-by-count":108,"title":["The <i>Legionella pneumophila<\/i> IcmS\u2013LvgA protein complex is important for Dot\/Icm\u2010dependent intracellular growth"],"prefix":"10.1111","volume":"61","author":[{"given":"Carr D.","family":"Vincent","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph 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