{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:18:43Z","timestamp":1791098323082,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,4]],"date-time":"2006-07-04T00:00:00Z","timestamp":1151971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Bubonic plague is transmitted by fleas whose feeding is blocked by a <jats:italic>Yersinia pestis<\/jats:italic> biofilm in the digestive tract. <jats:italic>Y.\u2003pestis<\/jats:italic> also block feeding of <jats:italic>Caenorhabditis elegans<\/jats:italic> by forming a biofilm on the nematode head, making the nematode an experimentally tractable surrogate for fleas to study plague transmission. Arabinose 5\u2010phosphate isomerase (API), encoded by <jats:italic>Y.\u2003pestis yrbH,<\/jats:italic> catalyses the conversion of ribulose 5\u2010phosphate into arabinose 5\u2010phosphate (A5P), the first committed step in the 3\u2010deoxy\u2010<jats:sc>d<\/jats:sc>\u2010<jats:italic>manno<\/jats:italic>\u2010oct\u20102\u2010ulosonic acid (Kdo) biosynthesis pathway. Here we show that <jats:italic>Y.\u2003pestis<\/jats:italic> YrbH is a multifunctional protein required for both Kdo biosynthesis and biofilm formation on <jats:italic>C.\u2003elegans.<\/jats:italic> The YrbH protein contains four functional components: biofilm\u2010related region 1 (B1), a sugar isomerase domain (SIS), biofilm\u2010related region 2 (B2) and a cystathionine \u03b2\u2010synthase domain pair (CBS). B1, SIS and B2 are all required for API function, but any of the three is sufficient for a biofilm\u2010related function. The CBS domain appears to negatively regulate the biofilm\u2010related function.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2006.05265.x","type":"journal-article","created":{"date-parts":[[2006,7,5]],"date-time":"2006-07-05T23:07:37Z","timestamp":1152140857000},"page":"861-870","source":"Crossref","is-referenced-by-count":31,"title":["<i>Yersinia pestis<\/i> YrbH is a multifunctional protein required for both 3\u2010deoxy\u2010<scp>d<\/scp>\u2010<i>manno<\/i>\u2010oct\u20102\u2010ulosonic acid biosynthesis and biofilm formation"],"prefix":"10.1111","volume":"61","author":[{"given":"Li","family":"Tan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Creg","family":"Darby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,4]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"423","article-title":"Observations on the mechanism of the transmission 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