{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T21:48:26Z","timestamp":1764193706524},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,12]],"date-time":"2006-07-12T00:00:00Z","timestamp":1152662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Listeria monocytogenes<\/jats:italic> is an intracytosolic bacterial pathogen that escapes from the phagosome using a secreted cytolysin, listeriolysin O (LLO). In the host cytosol, LLO activity is minimized to prevent pore formation in the host plasma membrane; premature lysis of the infected host cell exposes the bacteria to extracellular immune defences of the host and is detrimental to infection. Here we identified nucleotide substitutions in the coding sequence of the LLO gene (<jats:italic>hly<\/jats:italic>) that did not alter the protein sequence, yet caused over\u2010production of LLO, cytotoxicity and loss of virulence. These phenotypes were independent of the promoter and, under conditions in which the mutants produced more LLO protein than wild type, levels of <jats:italic>hly<\/jats:italic> mRNA were similar. Finally, negative regulation of LLO was maintained even when bacteria were engineered to produce elevated levels of the wild\u2010type <jats:italic>hly<\/jats:italic> transcript. Together, our data demonstrate that translational regulation of LLO is critical for <jats:italic>L. monocytogenes<\/jats:italic> pathogenesis.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2006.05286.x","type":"journal-article","created":{"date-parts":[[2006,7,12]],"date-time":"2006-07-12T12:44:27Z","timestamp":1152708267000},"page":"999-1012","source":"Crossref","is-referenced-by-count":46,"title":["Regulated translation of listeriolysin O controls virulence of <i>Listeria monocytogenes<\/i>"],"prefix":"10.1111","volume":"61","author":[{"given":"Pamela","family":"Schnupf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Hofmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Norseen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian J.","family":"Glomski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hal","family":"Schwartzstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy L.","family":"Decatur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90515-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/345175a0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.4049\/jimmunol.139.6.2005","article-title":"Adoptive transfer of immunity to Listeria monocytogenes. The influence of in vitro stimulation of lymphocyte subset requirements","volume":"139","author":"Bishop D.K.","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00390.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.6.1467"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.10.4688-4692.1999"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00008633"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01211.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.11.3355-3362.2004"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.57.11.3629-3636.1989"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.21.5935-5945.2002"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5493.992"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.57.030502.090934"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.55.11.2822-2829.1987"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.999-1003.2000"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200201081"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6754-6765.2003"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404924101"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90358-2"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00905-4"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.12.5608-5613.1994"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)36034-0"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.3.1291-1297.1987"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04252.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.15.4177-4186.2002"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.69.1.101-123.2005"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.21.8336"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03072.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2001.02281.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03176.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.1128\/aem.62.8.3057-3060.1996","article-title":"Modified Listeria bacteriophage lysin genes (ply) allow efficient overexpression and one\u2010step purification of biochemically active fusion proteins","volume":"62","author":"Loessner M.J.","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1403"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.6.1381"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb02158.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03413.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00436-1"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.1.131-139.1999"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.6.655"},{"key":"e_1_2_6_40_1","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.1128\/iai.59.12.4641-4646.1991","article-title":"Production and characterization of neutralizing and nonneutralizing monoclonal antibodies against LLO","volume":"59","author":"Nato F.","year":"1991","journal-title":"Infect Immun"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/353852a0"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.4.1459"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200205009"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.22.6573-6578.2001"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(96)10031-1"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.22.7174-7180.1997"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2876518"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00185-C"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00631.x"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04780.x"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.11.4231-4237.1995"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.20.5953-5958.2003"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2004.02.015"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.58.11.3770-3778.1990"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.6199-6209.2005"},{"key":"e_1_2_6_56_1","doi-asserted-by":"crossref","first-page":"5227","DOI":"10.4049\/jimmunol.155.11.5227","article-title":"Listeriolysin is processed efficiently into an MHC class I\u2010associated epitope in Listeria monocytogenes\u2010infected cells","volume":"155","author":"Villanueva M.S.","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00348.x"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00437-3"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.21.6052"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2958.2006.05286.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2958.2006.05286.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T08:01:14Z","timestamp":1695974474000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2958.2006.05286.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7,12]]},"references-count":58,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["10.1111\/j.1365-2958.2006.05286.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2958.2006.05286.x","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7,12]]}}}