{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:47:14Z","timestamp":1791010034832,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,8,23]],"date-time":"2006-08-23T00:00:00Z","timestamp":1156291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Entamoeba histolytica<\/jats:italic>\n                    is a protozoan intestinal parasite that causes amoebic colitis and amoebic liver abscess. To identify virulence factors of\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    , we first defined the phenotypes of two\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    strains, HM\u20101:IMSS, the prototype virulent strain, and\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    Rahman, a strain that was reportedly less virulent than HM\u20101:IMSS. We found that compared with HM\u20101:IMSS, Rahman has a defect in erythrophagocytosis and the ability to cause amoebic colitis in human colonic xenografts. We used differential in\u2010gel 2D electrophoresis to compare the proteome of Rahman and HM\u20101:IMSS, and identified six proteins that were differentially expressed above a fivefold level between the two organisms. These included two proteins with antioxidative properties (peroxiredoxin and superoxide dismutase), and three proteins of unknown function, grainin 1, grainin 2 and a protein containing a LIM\u2010domain. Overexpression of peroxiredoxin in Rahman rendered the transgenic trophozoites more resistant to killing by H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:italic>in vitro<\/jats:italic>\n                    , and infection with Rahman trophozoites expressing higher levels of peroxiredoxin was associated with higher levels of intestinal inflammation in human colonic xenografts, and more severe disease based on histology. In contrast, higher levels of grainin appear to be associated with a reduced virulence phenotype, and\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    HM\u20101:IMSS trophozoites infecting human intestinal xenografts show marked decreases in grainin expression. Our data indicate that there are definable molecular differences between Rahman and HM\u20101:IMSS that may explain the phenotypic differences, and identify peroxiredoxin as an important component of virulence in amoebic colitis.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2958.2006.05344.x","type":"journal-article","created":{"date-parts":[[2006,8,23]],"date-time":"2006-08-23T08:36:16Z","timestamp":1156322176000},"page":"1523-1532","source":"Crossref","is-referenced-by-count":98,"title":["Comparative proteomic analysis of two\n                    <i>Entamoeba histolytica<\/i>\n                    strains with different virulence phenotypes identifies peroxiredoxin as an important component of amoebic virulence"],"prefix":"10.1111","volume":"61","author":[{"given":"Paul H.","family":"Davis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Reid","family":"Townsend","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Samuel L.","family":"Stanley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,8,23]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2003.10.006"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402353101"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3260785"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02941.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(03)00268-6"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(88)90095-1"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2005.04.014"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00321.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(78)90144-X"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5769(02)00090-9"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T100007-MCP200"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2006.02.007"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.19.8975"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.775"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac026136s"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.1.01764"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1499"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac051520l"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2004.03.007"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0188-4409(00)00145-4"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.5.1725"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.340-351.2006"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01178"},{"key":"e_1_2_5_25_1","doi-asserted-by":"crossref","first-page":"882","DOI":"10.4269\/ajtmh.1978.27.882","article-title":"Experimental amebiasis. 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