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We developed an <jats:italic>in vivo<\/jats:italic> expression technology (IVET), with an insect host, for identification of the <jats:italic>B.\u2003cereus<\/jats:italic> genes specifically expressed during infection. This IVET\u2010based approach uses site\u2010specific recombinase TnpI to identify transient promoter activation. We constructed a genomic library of <jats:italic>B.\u2003cereus<\/jats:italic> ATCC14579 by cloning DNA fragments upstream from <jats:italic>tnpI<\/jats:italic>. The library was screened <jats:italic>in vivo<\/jats:italic> by oral infection of the insect <jats:italic>Galleria mellonella<\/jats:italic>. We selected 100 clones from dead larvae. Sequencing of the inserts followed by a second screen for specific <jats:italic>in vivo<\/jats:italic> induction led to the identification of 20 <jats:italic>in vivo<\/jats:italic>\u2010induced genes (<jats:italic>ivi<\/jats:italic> genes). They belonged to several different functional classes: regulation, metabolism, DNA repair and replication, cell division, transport, virulence and adaptation. A strongly induced gene, <jats:italic>ivi29<\/jats:italic>, was further analysed. It encodes an internalin\u2010like protein with four distinct domains: an N\u2010terminal signal peptide for export, a NEAT domain thought to be involved in iron transport, a leucine\u2010rich repeat domain that may interact with host cells, and a C\u2010terminal SLH domain presumably binding the protein to the peptidoglycan. As suggested by a Fur box in the promoter, transcriptional analysis showed <jats:italic>ivi29<\/jats:italic> expression to be repressed by iron, suggesting that expression was induced <jats:italic>in vivo<\/jats:italic> due to iron deprivation in the host. This iron\u2010regulated, leucine\u2010rich surface protein was designated IlsA. Disruption of <jats:italic>ilsA<\/jats:italic> reduced the virulence of the bacteria to the insect larvae indicating its role in the overall pathogenesis of <jats:italic>B.\u2003cereus<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2006.05362.x","type":"journal-article","created":{"date-parts":[[2006,9,15]],"date-time":"2006-09-15T15:05:52Z","timestamp":1158332752000},"page":"339-355","source":"Crossref","is-referenced-by-count":95,"title":["Identification of <i>Bacillus cereus<\/i> internalin and other candidate virulence genes specifically induced during oral infection in insects"],"prefix":"10.1111","volume":"62","author":[{"given":"Sinda","family":"Fedhila","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Daou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Lereclus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Nielsen\u2010LeRoux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,9,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00405.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01419.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-9-research0047"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90495-W"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601629"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.11.4423-4428.1995"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1994.1094"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02798.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199911)21:11<932::AID-BIES5>3.0.CO;2-N"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8903-8910.2005"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)90365-4"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1007\/BF02172985","article-title":"Analysis of cryIAa expression in sigE and sigK mutants of Bacillus thuringiensis","volume":"250","author":"Bravo A.","year":"1996","journal-title":"Mol Gen Genet"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2002.tb00617.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.7.3348-3356.1999"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3116-3124.2003"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.7.2634"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03659.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2003.10.007"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.13.6127"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.65.5.1615-1625.1997"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.20.6223-6229.1999"},{"key":"e_1_2_6_23_1","unstructured":"Fedhila S.(2002)Contribution des m\u00e9talloprot\u00e9ases InhA et des prot\u00e9ines de choc thermique Clp \u00e0 la virulence deBacillus thuringiensis. 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