{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:45:12Z","timestamp":1791103512433,"version":"4.1.0"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,9,27]],"date-time":"2006-09-27T00:00:00Z","timestamp":1159315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>(1\u21923)\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010Glucans are major components of the cell walls of Oomycetes and as such they play an essential role in the morphogenesis and growth of these microorganisms. Despite the biological importance of (1\u21923)\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucans, their mechanisms of biosynthesis are poorly understood. Previous studies on (1\u21923)\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucan synthases from <jats:italic>Saprolegnia monoica<\/jats:italic> have shown that three protein bands of an apparent molecular weight of 34, 48 and 50\u2003kDa co\u2010purify with enzyme activity. However, none of the corresponding proteins have been identified. Here we have identified, purified, sequenced and characterized a protein from the 34\u2003kDa band and clearly shown that it has all the biochemical properties of proteins from the annexin family. In addition, we have unequivocally demonstrated that the purified protein is an activator of (1\u21923)\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucan synthase. This represents a new type of function for proteins belonging to the annexin family. Two other proteins from the 48 and 50\u2003kDa bands were identified as ATP synthase subunits, which most likely arise from contaminations by mitochondria during membrane preparation. The results, which are discussed in relation with the possible regulation mechanisms of (1\u21923)\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucan synthases, represent a first step towards a better understanding of cell wall polysaccharide biosynthesis in Oomycetes.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2006.05389.x","type":"journal-article","created":{"date-parts":[[2006,9,15]],"date-time":"2006-09-15T15:05:52Z","timestamp":1158332752000},"page":"552-565","source":"Crossref","is-referenced-by-count":23,"title":["Identification of the first Oomycete annexin as a (1\u21923)\u2010\u03b2\u2010<scp>d<\/scp>\u2010glucan synthase activator"],"prefix":"10.1111","volume":"62","author":[{"given":"Jamel","family":"Bouzenzana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovic","family":"Pelosi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Briolay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Briolay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Bulone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,9,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.1993.00763.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/00275514.1978.12020236"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5493.972"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-1878(200102)23:2<170::AID-BIES1024>3.0.CO;2-#"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.22.100168.000511"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1991.tb16076.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.7.2273-2279.2001"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-10-3175"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150080203"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)80582-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00006409"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08328.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.94.4.1748"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0147-5975(92)90037-R"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1995.08020213.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(89)90038-9"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.8.2.333"},{"key":"e_1_2_6_20_1","first-page":"207","article-title":"Isolation and characterization of chitin from the cell walls of Achlya radiosa","volume":"128","author":"Campos\u2010Takaki G.M.","year":"1982","journal-title":"J Gen Microbiol"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.1993.10508696"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.50.1.245"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050070"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcf164"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.26.12907"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00392983"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS.1997.81.12.1349"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.2307\/1313151"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj119"},{"key":"e_1_2_6_31_1","first-page":"543","article-title":"SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny","volume":"12","author":"Galtier N.","year":"1996","journal-title":"Comput Appl Biosci"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/320636a0"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00030.2001"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9452(91)90141-T"},{"key":"e_1_2_6_35_1","first-page":"51","article-title":"Annexins in the plant kingdoms","volume":"1","author":"Hofmann A.","year":"2004","journal-title":"Annexins"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03612.x"},{"key":"e_1_2_6_37_1","unstructured":"Johnson T.W. 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