{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:45:41Z","timestamp":1791078341200,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,10,13]],"date-time":"2006-10-13T00:00:00Z","timestamp":1160697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>IS<jats:italic>911<\/jats:italic> naturally produces transposase (OrfAB) derivatives truncated at the C\u2010terminal end (OrfAB\u2010CTF) and devoid of the catalytic domain. A majority species, OrfAB*, was produced at higher levels at 42\u00b0C than at 30\u00b0C suggesting that it is at least partly responsible for the innate reduction in IS<jats:italic>911<\/jats:italic> transposition activity at higher temperatures. An engineered equivalent of similar length, OrfAB[1\u2013149], inhibited transposition activity <jats:italic>in vivo<\/jats:italic> or <jats:italic>in vitro<\/jats:italic> when produced along with full\u2010length transposase. We isolated several point mutants showing higher activity than the wild\u2010type IS<jats:italic>911<\/jats:italic> at 42\u00b0C. These fall into two regions of the transposase. One, located in the N\u2010terminal segment of OrfAB, lies between or within two regions involved in protein multimerization. The other is located within the C\u2010terminal catalytic domain. The N\u2010terminal mutations resulted in reduced levels of OrfAB* while the C\u2010terminal mutation alone appeared not to affect OrfAB* levels. Combination of N\u2010 and C\u2010terminal mutations greatly reduced OrfAB* levels and transposition was concomitantly high even at 42\u00b0C. The mechanism by which truncated transposase species are generated and how they intervene to reduce transposition activity is discussed. While transposition activity of these multiply mutated derivatives <jats:italic>in vivo<\/jats:italic> was resistant to temperature, the purified OrfAB derivatives retained an inherent temperature\u2010sensitive phenotype <jats:italic>in vitro<\/jats:italic>. This clearly demonstrates that temperature sensitivity of IS<jats:italic>911<\/jats:italic> transposition is a complex phenomenon with several mechanistic components. These results have important implications for the several other transposons and insertion sequences whose transposition has also been shown to be temperature\u2010sensitive.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2006.05424.x","type":"journal-article","created":{"date-parts":[[2006,10,14]],"date-time":"2006-10-14T20:37:24Z","timestamp":1160858244000},"page":"1102-1116","source":"Crossref","is-referenced-by-count":18,"title":["Truncated forms of IS<i>911<\/i> transposase downregulate 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