{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:13:58Z","timestamp":1792739638110,"version":"4.5.0"},"reference-count":71,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,1,8]],"date-time":"2007-01-08T00:00:00Z","timestamp":1168214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The genome of the Lyme disease\u2010causing spirochete <jats:italic>Borrelia burgdorferi<\/jats:italic> encodes only a single polypeptide from the integration host factor (IHF)\/HU or \u2018DNABII\u2019 family of nucleoid\u2010associated proteins \u2013 Hbb. DNABII proteins induce large bends in DNA and serve as architectural factors in a variety of prokaryotic cellular processes. We have solved the crystal structure of an Hbb\u2013DNA complex in which the DNA is bent by over 180\u00b0. We find that like IHF, Hbb relies exclusively on indirect readout to recognize its cognate site. Additional binding studies show that the sequence preferences of Hbb are related to, yet distinct from those of IHF. Defining these binding characteristics may help to uncover additional roles for Hbb in <jats:italic>Borrelia<\/jats:italic> DNA metabolism as well as further our understanding of the mechanism of indirect readout.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2007.05586.x","type":"journal-article","created":{"date-parts":[[2007,1,22]],"date-time":"2007-01-22T18:57:24Z","timestamp":1169492244000},"page":"1319-1330","source":"Crossref","is-referenced-by-count":68,"title":["Shaping the <i>Borrelia burgdorferi<\/i> genome: crystal structure and binding properties of the DNA\u2010bending protein Hbb"],"prefix":"10.1111","volume":"63","author":[{"given":"Kent W.","family":"Mouw","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phoebe 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