{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:01:27Z","timestamp":1790406087094,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,1,15]],"date-time":"2007-01-15T00:00:00Z","timestamp":1168819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Enterohaemorrhagic <jats:italic>Escherichia coli<\/jats:italic> (EHEC) are highly infectious pathogens capable of causing severe diarrhoeal illnesses. As a critical step during their colonization, EHEC adhere intimately to intestinal epithelial cells and generate F\u2010actin \u2018pedestal\u2019 structures that elevate them above surrounding cell surfaces. Intimate adhesion and pedestal formation result from delivery of the EHEC type III secretion system (TTSS) effector proteins Tir and EspF<jats:sub>U<\/jats:sub> into the host cell and expression of the bacterial outer membrane adhesin, intimin. To investigate a role for DNA methylation during the regulation of adhesion and pedestal formation in EHEC, we deleted the <jats:italic>dam<\/jats:italic> (<jats:styled-content>D<\/jats:styled-content>NA <jats:styled-content>a<\/jats:styled-content>denine <jats:styled-content>m<\/jats:styled-content>ethyltransferase) gene from EHEC O157:H7 and demonstrate that this mutation results in increased interactions with cultured host cells. EHEC\u0394<jats:italic>dam<\/jats:italic> exhibits dramatically elevated levels of adherence and pedestal formation when compared with wild\u2010type EHEC, and expresses significantly higher protein levels of intimin, Tir and EspF<jats:sub>U<\/jats:sub>. Analyses of GFP fusions, Northern blotting, reverse transcription polymerase chain reaction, and microarray experiments indicate that the abundance of Tir in the <jats:italic>dam<\/jats:italic> mutant is not due to increased transcription levels, raising the possibility that Dam methylation can indirectly control protein expression by a post\u2010transcriptional mechanism. In contrast to other <jats:italic>dam<\/jats:italic>\u2010deficient pathogens, EHEC\u0394<jats:italic>dam<\/jats:italic> is capable of robust intestinal colonization of experimentally infected animals.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2007.05602.x","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T03:59:42Z","timestamp":1171511982000},"page":"1468-1481","source":"Crossref","is-referenced-by-count":50,"title":["Increased adherence and actin pedestal formation by <i>dam<\/i>\u2010deficient enterohaemorrhagic <i>Escherichia coli<\/i> O157:H7"],"prefix":"10.1111","volume":"63","author":[{"given":"Kenneth G.","family":"Campellone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Roe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"L\u00f8bner\u2010Olesen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenan C.","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loranne","family":"Magoun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Brady","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Donohue\u2010Rolfe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saul","family":"Tzipori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Gally","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Leong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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