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Dissemination occurs through the circulation and through tissue matrices, but the <jats:italic>B.\u2003burgdorferi<\/jats:italic> molecules that mediate interactions with the endothelium <jats:italic>in vivo<\/jats:italic> have not yet been identified. <jats:italic>In vivo<\/jats:italic> selection of filamentous phage expressing <jats:italic>B.\u2003burgdorferi<\/jats:italic> protein fragments on the phage surface identified several new candidate adhesins, and verified the activity of one adhesin that had been previously characterized <jats:italic>in vitro<\/jats:italic>. P66, a <jats:italic>B.\u2003burgdorferi<\/jats:italic> ligand for \u03b2<jats:sub>3<\/jats:sub>\u2010chain integrins, OspC, a protein that is essential for the establishment of infection in mammals, and Vls, a protein that undergoes antigenic variation in the mammal, were all selected for binding to the murine endothelium <jats:italic>in vivo<\/jats:italic>. Additional <jats:italic>B.\u2003burgdorferi<\/jats:italic> proteins for which no functions have been identified, including all four members of the OspF family and BmpD, were identified as candidate adhesins. The use of <jats:italic>in vivo<\/jats:italic> phage display is one approach to the identification of adhesins in pathogenic bacteria that are not easily grown in the laboratory, or for which genetic manipulations are not straightforward.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2007.05924.x","type":"journal-article","created":{"date-parts":[[2007,9,7]],"date-time":"2007-09-07T12:25:04Z","timestamp":1189167904000},"page":"262-276","source":"Crossref","is-referenced-by-count":85,"title":["<i>Borrelia burgdorferi<\/i> adhesins identified using <i>in vivo<\/i> phage display"],"prefix":"10.1111","volume":"66","author":[{"given":"Styliani","family":"Antonara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca M.","family":"Chafel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"LaFrance","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenifer","family":"Coburn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,8,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_18030507.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.7.3847"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00325-4"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5349.377"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0202-121"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1992.47.249"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.50.4.381-400.1986"},{"key":"e_1_2_5_9_1","first-page":"263","article-title":"Kinetics of Borrelia burgdorferi dissemination and evolution of disease after intradermal inoculation of mice","volume":"139","author":"Barthold S.W.","year":"1991","journal-title":"Am J Pathol"},{"key":"e_1_2_5_10_1","first-page":"959","article-title":"Chronic Lyme borreliosis in the laboratory mouse","volume":"143","author":"Barthold S.W.","year":"1993","journal-title":"Am J Pathol"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03557.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3371-3383.2003"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.296-304.2006"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11692"},{"key":"e_1_2_5_15_1","first-page":"935","article-title":"Borrelia burgdorferi BmpA, BmpB, and BmpD proteins are expressed in human infection and contribute to P39 immunoblot reactivity in patients with Lyme disease","volume":"12","author":"Bryksin A.V.","year":"2005","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26944-0"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02046-7"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01698.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/352624a0"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05224.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01654.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.3455-3459.2001"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.12.3734"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010062200"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201547200"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.2139-2150.2002"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1159\/000073406"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.435-441.2006"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3138-3145.2003"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5938-5946.2004"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306845101"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.9.3467-3472.1995"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00012a012"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.9.5042-5055.2003"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.15.4133"},{"key":"e_1_2_5_37_1","first-page":"26","article-title":"Lyme arthritis. 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