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Bacterial life within the host requires the expression of genes with roles in nutrient acquisition as well as the biosynthesis of bacterial products required to support intracellular growth. A gene product identified as the substrate\u2010binding component of a novel oligopeptide transport system (encoded by\n                    <jats:italic>lmo0135<\/jats:italic>\n                    ) was recently shown to be required for\n                    <jats:italic>L.\u2003monocytogenes<\/jats:italic>\n                    virulence. Here we demonstrate that\n                    <jats:italic>lmo0135<\/jats:italic>\n                    encodes a multifunctional protein that is associated with cysteine transport, acid resistance, bacterial membrane integrity and adherence to host cells. The\n                    <jats:italic>lmo0135<\/jats:italic>\n                    gene product (designated CtaP, for\n                    <jats:styled-content>c<\/jats:styled-content>\n                    ysteine\n                    <jats:styled-content>t<\/jats:styled-content>\n                    ransport\n                    <jats:styled-content>a<\/jats:styled-content>\n                    ssociated\n                    <jats:styled-content>p<\/jats:styled-content>\n                    rotein) was required for bacterial growth in the presence of low concentrations of cysteine\n                    <jats:italic>in vitro<\/jats:italic>\n                    , but was not required for bacterial replication within the host cytosol. Loss of CtaP increased membrane permeability and acid sensitivity, and reduced bacterial adherence to host cells.\n                    <jats:italic>ctaP<\/jats:italic>\n                    deletion mutants were severely attenuated following intragastric and intravenous inoculation of mice. Taken together, the data presented indicate that CtaP contributes to multiple facets of\n                    <jats:italic>L.\u2003monocytogenes<\/jats:italic>\n                    physiology, growth and survival both inside and outside of animal cells.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2958.2009.06910.x","type":"journal-article","created":{"date-parts":[[2009,10,8]],"date-time":"2009-10-08T06:52:17Z","timestamp":1254984737000},"page":"956-973","source":"Crossref","is-referenced-by-count":60,"title":["<i>Listeria monocytogenes<\/i>\n                    CtaP is a multifunctional cysteine transport\u2010associated protein required for bacterial pathogenesis"],"prefix":"10.1111","volume":"74","author":[{"given":"Bobbi","family":"Xayarath","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Marquis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary C.","family":"Port","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy E.","family":"Freitag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,11,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00904.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00280-09"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1128\/jb.131.1.111-118.1977","article-title":"Regulation of 1\u2010cystine transport in Salmonella typhimurium","volume":"131","author":"Baptist E.W.","year":"1977","journal-title":"J Bacteriol"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00976-06"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"7684","DOI":"10.1016\/S0021-9258(19)44579-1","article-title":"A binding protein involved in the transport of cystine and diaminopimelic acid in Escherichia coli","volume":"247","author":"Berger E.A.","year":"1972","journal-title":"J Biol Chem"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1128\/aem.60.4.1359-1363.1994","article-title":"Effect of exogenous proline, betaine, and carnitine on growth of Listeria monocytogenes in a minimal medium","volume":"60","author":"Beumer R.R.","year":"1994","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.4049\/jimmunol.139.6.2005","article-title":"Adoptive transfer of immunity to Listeria monocytogenes. 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