{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:59:23Z","timestamp":1790985563631,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,9,30]],"date-time":"2010-09-30T00:00:00Z","timestamp":1285804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Vibrio cholerae<\/jats:italic> causes the severe diarrhoeal disease cholera. A cascade of regulators controls expression of virulence determinants in <jats:italic>V. cholerae<\/jats:italic> at both transcriptional and post\u2010transcriptional levels. ToxT is the direct transcription activator of the major virulence genes in <jats:italic>V. cholerae<\/jats:italic>. Here we describe TarA, a highly conserved, small regulatory RNA, whose transcription is activated by ToxT from toxboxes present upstream of the ToxT\u2010activated gene <jats:italic>tcpI<\/jats:italic>. TarA regulates <jats:italic>ptsG<\/jats:italic>, encoding a major glucose transporter in <jats:italic>V. cholerae<\/jats:italic>. Cells overexpressing TarA exhibit decreased steady\u2010state levels of <jats:italic>ptsG<\/jats:italic> mRNA and grow poorly in glucose\u2010minimal media. A mutant lacking the ubiquitous regulatory protein Hfq expresses diminished TarA levels, indicating that TarA likely interacts with Hfq to regulate gene expression. RNAhybrid analysis of TarA and the putative <jats:italic>ptsG<\/jats:italic> mRNA leader suggests potential productive base\u2010pairing between these two RNA molecules. A <jats:italic>V. cholerae<\/jats:italic> mutant lacking TarA is compromised for infant mouse colonization in competition with wild type, suggesting a role in the <jats:italic>in vivo<\/jats:italic> fitness of <jats:italic>V. cholerae<\/jats:italic>. Although somewhat functionally analogous to SgrS of <jats:italic>Escherichia coli<\/jats:italic>, TarA does not encode a regulatory peptide, and its expression is activated by the virulence gene pathway in <jats:italic>V. cholerae<\/jats:italic> and not by glycolytic intermediates.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2010.07397.x","type":"journal-article","created":{"date-parts":[[2010,9,27]],"date-time":"2010-09-27T12:50:21Z","timestamp":1285591821000},"page":"1171-1181","source":"Crossref","is-referenced-by-count":51,"title":["The <i>Vibrio cholerae<\/i> virulence regulatory cascade controls glucose uptake through activation of TarA, a small regulatory RNA"],"prefix":"10.1111","volume":"78","author":[{"given":"Aimee L.","family":"Richard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey 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