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DsbD provides reducing power to DsbC that shuffles incorrect disulfide bond in misfolded proteins as well as to the periplasmic enzymes that reduce apo\u2010cytochrome <jats:italic>c<\/jats:italic> (CcsX) or repair oxidative protein damages (MrsAB). The expression of <jats:italic>dsbD<\/jats:italic>, but not other <jats:italic>dsb<\/jats:italic> genes, is positively regulated by the MisR\/S two\u2010component system. Quantitative real\u2010time PCR analyses showed significantly reduced <jats:italic>dsbD<\/jats:italic> expression in all <jats:italic>misR\/S<\/jats:italic> mutants, which was rescued by genetic complementation. The direct and specific interaction of MisR with the upstream region of the <jats:italic>dsbD<\/jats:italic> promoter was demonstrated by electrophoretic mobility shift assay, and the MisR binding sequences were mapped. Further, the expression of <jats:italic>dsbD<\/jats:italic> was found to be induced by dithiothrietol (DTT), through the MisR\/S regulatory system. Surprisingly, we revealed that inactivation of <jats:italic>dsbD<\/jats:italic> can only be achieved in a strain carrying an ectopically located <jats:italic>dsbD<\/jats:italic>, in the <jats:italic>dsbA1A2<\/jats:italic> double mutant or in the <jats:italic>dsbA1A2A3<\/jats:italic> triple mutant, thus DsbD is indispensable for DsbA\u2010catalysed oxidative protein folding in <jats:italic>N. meningitidis<\/jats:italic>. The defects of the meningococcal <jats:italic>dsbA1A2<\/jats:italic> mutant in transformation and resistance to oxidative stress were more severe in the absence of <jats:italic>dsbD<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07546.x","type":"journal-article","created":{"date-parts":[[2011,1,10]],"date-time":"2011-01-10T13:08:04Z","timestamp":1294664884000},"page":"1557-1573","source":"Crossref","is-referenced-by-count":26,"title":["Characterization of DsbD in <i>Neisseria meningitidis<\/i>"],"prefix":"10.1111","volume":"79","author":[{"given":"Pradeep","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soma","family":"Sannigrahi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Scoullar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlene M.","family":"Kahler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yih\u2010Ling","family":"Tzeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,1,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00714-09"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001055"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02174.x"},{"key":"e_1_2_6_5_1","first-page":"1","article-title":"Prevention and control of meningococcal disease. 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