{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T12:43:04Z","timestamp":1788093784787,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,3,31]],"date-time":"2011-03-31T00:00:00Z","timestamp":1301529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Secreted phospholipase B1 (CnPlb1) is essential for dissemination of <jats:italic>Cryptococcus neoformans<\/jats:italic> to the central nervous system (CNS) yet essential components of its secretion machinery remain to be elucidated. Using gene deletion analysis we demonstrate that CnPlb1 secretion is dependent on the Cn<jats:italic>SEC14<\/jats:italic> product, CnSec14\u20101p. CnSec14\u20101p is a homologue of the phosphatidylinositol transfer protein ScSec14p, which is essential for secretion and viability in <jats:italic>Saccharomyces cerevisiae<\/jats:italic>. In contrast to CnPlb1, neither laccase 1\u2010induced melanization within the cell wall nor capsule induction were negatively impacted in Cn<jats:italic>SEC14\u20101<\/jats:italic> deletion mutants (Cn<jats:italic>\u0394sec14\u20101<\/jats:italic> and Cn<jats:italic>\u0394sec14\u20101<\/jats:italic>Cn\u0394<jats:italic>sfh5<\/jats:italic>). Similar to the Cn<jats:italic>PLB1<\/jats:italic> deletion mutant (Cn<jats:italic>\u0394plb1<\/jats:italic>), Cn<jats:italic>\u0394sec14\u20101<\/jats:italic> was hypovirulent in mice and did not disseminate to the CNS by day 14 post infection. Furthermore, macrophage expulsion of live Cn<jats:italic>\u0394sec14\u20101<\/jats:italic> and Cn<jats:italic>\u0394plb1<\/jats:italic> (vomocytosis) was reduced. Individual deletion of Cn<jats:italic>SEC14\u20102<\/jats:italic>, a closely related Cn<jats:italic>SEC14\u20101<\/jats:italic> homologue, and Cn<jats:italic>SFH5<\/jats:italic>, a distantly related SEC fourteen like homologue, did not abrogate CnPlb1 secretion or virulence. However, reconstitution of Cn\u0394<jats:italic>sec14\u20101<\/jats:italic> with Cn<jats:italic>SEC14\u20101<\/jats:italic> or Cn<jats:italic>SEC14\u20102<\/jats:italic> restored both phenotypes, consistent with functional genetic redundancy. We conclude that CnPlb1 secretion is <jats:italic>SEC14\u2010<\/jats:italic>dependent and that <jats:italic>C. neoformans<\/jats:italic> preferentially exports virulence determinants to the cell periphery via distinct pathways. We also demonstrate that CnPlb1 secretion is essential for vomocytosis.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07632.x","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T11:13:25Z","timestamp":1301656405000},"page":"1088-1101","source":"Crossref","is-referenced-by-count":94,"title":["<i>SEC14<\/i> is a specific requirement for secretion of phospholipase B1 and pathogenicity of <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1111","volume":"80","author":[{"given":"Methee","family":"Chayakulkeeree","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon Andrew","family":"Johnston","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanes Bijosono","family":"Oei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Lev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter Richard","family":"Williamson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christabel Frewen","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Lusia","family":"Leal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilene Henning","family":"Vainstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wieland","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania Christine","family":"Sorrell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin Charles","family":"May","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julianne Teresa","family":"Djordjevic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,3,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2006.09.061"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-8-16"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.3254"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.4.1271"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2009.10.008"},{"key":"e_1_2_6_7_1","volume-title":"Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual","author":"Burke D.","year":"2000"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00857.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01065-08"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06310.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3470431"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02236.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808732200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-8-2607"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050063"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/10.Supplement_2.S274"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0648r.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.10.018"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.131.2.297"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32053"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001041"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1583"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200901145"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2006.09.032"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-8-15"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.1.202-208.2005"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(19960915)12:11<1097::AID-YEA990>3.0.CO;2-E"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.1.10"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsyr.2004.04.004"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06588.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e328322ffac"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2008.08.003"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2229-2239.2004"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-48-8-731"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780412331282331"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03688.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02236.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707913200"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110.21.2703"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2006.07.002"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0029(20001215)51:6<530::AID-JEMT4>3.0.CO;2-Q"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(02)00024-2"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00262-06"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E06-08-0701"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2164(09)01204-0"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5589-5596.2001"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2958.2011.07632.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2958.2011.07632.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T19:34:20Z","timestamp":1697312060000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2958.2011.07632.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,31]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,5]]}},"alternative-id":["10.1111\/j.1365-2958.2011.07632.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2958.2011.07632.x","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3,31]]}}}