{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T01:51:40Z","timestamp":1792201900912,"version":"4.4.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,5,2]],"date-time":"2011-05-02T00:00:00Z","timestamp":1304294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Following translocation, bacterial lipoproteins are lipidated by lipoprotein diacylglycerol transferase (Lgt) and cleaved of their signal peptides by lipoprotein signal peptidase (Lsp). In Gram\u2010negative bacteria and mycobacteria, lipoproteins are further lipidated by lipoprotein N\u2010acyl transferase (Lnt), to give triacylated lipoproteins. <jats:italic>Streptomyces<\/jats:italic> are unusual amongst Gram\u2010positive bacteria because they export large numbers of lipoproteins via the twin arginine protein transport (Tat) pathway. Furthermore, some <jats:italic>Streptomyces<\/jats:italic> species encode two Lgt homologues and all <jats:italic>Streptomyces<\/jats:italic> species encode two homologues of Lnt. Here we characterize lipoprotein biogenesis in the plant pathogen <jats:italic>Streptomyces scabies<\/jats:italic> and report that <jats:italic>lgt<\/jats:italic> and <jats:italic>lsp<\/jats:italic> mutants are defective in growth and development while only moderately affected in virulence. Lipoproteins are lost from the membrane in an <jats:italic>S. scabies lgt<\/jats:italic> mutant but restored by expression of <jats:italic>Streptomyces coelicolor lgt1<\/jats:italic> or <jats:italic>lgt2<\/jats:italic> confirming that both encode functional Lgt enzymes. Furthermore, lipoproteins are N\u2010acylated in <jats:italic>Streptomyces<\/jats:italic> with efficient N\u2010acylation dependent on Lnt1 and Lnt2. However, deletion of <jats:italic>lnt1<\/jats:italic> and <jats:italic>lnt2<\/jats:italic> has no effect on growth, development or virulence. We thus present a detailed study of lipoprotein biogenesis in <jats:italic>Streptomyces<\/jats:italic>, the first study of Lnt function in a monoderm bacterium and the first study of bacterial lipoproteins as virulence factors in a plant pathogen.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07656.x","type":"journal-article","created":{"date-parts":[[2011,4,8]],"date-time":"2011-04-08T04:30:35Z","timestamp":1302237035000},"page":"1395-1412","source":"Crossref","is-referenced-by-count":46,"title":["Dissecting the complete lipoprotein biogenesis pathway in <i>Streptomyces scabies<\/i>"],"prefix":"10.1111","volume":"80","author":[{"given":"David A.","family":"Widdick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew G.","family":"Hicks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin 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