{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:24:44Z","timestamp":1790529884172,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,4,20]],"date-time":"2011-04-20T00:00:00Z","timestamp":1303257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Aminoglycoside resistance in bacteria can be acquired by several mechanisms, including drug modification, target alteration, reduced uptake and increased efflux. Here we demonstrate that increased resistance to the aminoglycosides streptomycin and spectinomycin in\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    can be conferred by increased expression of an aminoglycoside adenyl transferase encoded by the cryptic, chromosomally located\n                    <jats:italic>aadA<\/jats:italic>\n                    gene. During growth in rich medium the wild\u2010type strain was susceptible but mutations that impaired electron transport and conferred a small colony variant (SCV) phenotype or growth in glucose\/glycerol minimal media resulted in activation of the\n                    <jats:italic>aadA<\/jats:italic>\n                    gene and aminoglycoside resistance. Expression of the\n                    <jats:italic>aadA<\/jats:italic>\n                    gene was positively regulated by the stringent response regulator guanosine penta\/tetraphosphate ((p)ppGpp). SCV mutants carrying stop codon mutations in the\n                    <jats:italic>hemA<\/jats:italic>\n                    and\n                    <jats:italic>ubiA<\/jats:italic>\n                    genes showed a streptomycin pseudo\u2010dependent phenotype, where growth was stimulated by streptomycin. Our data suggest that this phenotype is due to streptomycin\u2010induced readthrough of the stop codons, a resulting increase in HemA\/UbiA levels and improved electron transport and growth. Our results demonstrate that environmental and mutational activation of a cryptic resistance gene can confer clinically significant resistance and that a streptomycin\u2010pseudo\u2010dependent phenotype can be generated via a novel mechanism that does not involve the classical\n                    <jats:italic>rpsL<\/jats:italic>\n                    mutations.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07657.x","type":"journal-article","created":{"date-parts":[[2011,4,20]],"date-time":"2011-04-20T22:40:11Z","timestamp":1303339211000},"page":"1464-1478","source":"Crossref","is-referenced-by-count":111,"title":["Activation of cryptic aminoglycoside resistance in\n                    <i>Salmonella enterica<\/i>"],"prefix":"10.1111","volume":"80","author":[{"given":"Sanna","family":"Koskiniemi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Pr\u00e4nting","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Gullberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joakim","family":"N\u00e4svall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan 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