{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T22:38:56Z","timestamp":1792449536025,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,5,5]],"date-time":"2011-05-05T00:00:00Z","timestamp":1304553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Pathogenic microorganisms like <jats:italic>Vibrio cholerae<\/jats:italic> are capable of adapting to diverse living conditions, especially when they transit from their environmental reservoirs to human host. <jats:italic>V. cholerae<\/jats:italic> attaches to N\u2010acetylglucosamine (GlcNAc) residues in glycoproteins and lipids present in the intestinal epithelium and chitinous surface of zoo\u2010phytoplanktons in the aquatic environment for its survival and colonization. GlcNAc utilization thus appears to be important for the pathogen to reach sufficient titres in the intestine for producing clinical symptoms of cholera. We report here the involvement of a second cluster of genes working in combination with the classical genes of GlcNAc catabolism, suggesting the occurrence of a novel variant of the process of biochemical conversion of GlcNAc to Fructose\u20106\u2010phosphate as has been described in other organisms. Colonization was severely attenuated in mutants that were incapable of utilizing GlcNAc. It was also shown that N\u2010acetylglucosamine specific repressor (NagC) performs a dual role \u2013 while the classical GlcNAc catabolic genes are under its negative control, the genes belonging to the second cluster are positively regulated by it. Further application of tandem affinity purification to NagC revealed its interaction with a novel partner. Our results provide a genetic program that probably enables <jats:italic>V. cholerae<\/jats:italic> to successfully utilize amino \u2013 sugars and also highlights a new mode of transcriptional regulation, not described in this organism.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07664.x","type":"journal-article","created":{"date-parts":[[2011,4,14]],"date-time":"2011-04-14T09:27:47Z","timestamp":1302773267000},"page":"1549-1560","source":"Crossref","is-referenced-by-count":43,"title":["Two gene clusters co\u2010ordinate for a functional N\u2010acetylglucosamine catabolic pathway in <i>Vibrio cholerae<\/i>"],"prefix":"10.1111","volume":"80","author":[{"given":"Swagata","family":"Ghosh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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