{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:16:04Z","timestamp":1791400564756,"version":"4.3.3"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,6,5]],"date-time":"2011-06-05T00:00:00Z","timestamp":1307232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Rrp1 is the sole c\u2010di\u2010GMP\u2010producing protein (diguanylate cyclase) of <jats:italic>Borrelia burgdorferi<\/jats:italic>. To test the hypothesis that Rrp1 regulates critical processes involved in the transmission of spirochetes between ticks and mammals, an <jats:italic>rrp1<\/jats:italic> deletion mutant (B31\u2010\u0394<jats:italic>rrp1<\/jats:italic>) and a strain that constitutively produces elevated levels of Rrp1 (B31\u2010OV) were constructed. The strains were assessed for progression through the enzootic cycle using an <jats:italic>Ixodes<\/jats:italic> tick\/C3H\u2010HeJ mouse model and tick immersion feeding methods. B31\u2010\u0394<jats:italic>rrp1<\/jats:italic> infected mice as efficiently as wild type but had altered motility, decreased chemotactic responses to <jats:italic>N<\/jats:italic>\u2010acetylglucosamine (NAG) and attenuated ability to disseminate or colonize distal organs. While this strain infected mice, it was not able to survive in ticks. In contrast, B31\u2010OV displayed normal motility patterns and chemotactic responses but was non\u2010infectious in mice. Using immersion feeding techniques, we demonstrate that B31\u2010OV can establish a population in ticks and survive exposure to a natural bloodmeal. The results presented here indicate Rrp1, and by extension, c\u2010di\u2010GMP, are not strictly required for murine infection, but are required for the successful establishment of a productive population of <jats:italic>B. burgdorferi<\/jats:italic> in ticks. These analyses provide significant new insight into the genetic regulatory mechanisms of the Lyme disease spirochetes.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07687.x","type":"journal-article","created":{"date-parts":[[2011,5,4]],"date-time":"2011-05-04T10:21:40Z","timestamp":1304504500000},"page":"219-231","source":"Crossref","is-referenced-by-count":83,"title":["The diguanylate cyclase, Rrp1, regulates critical steps in the enzootic cycle of the Lyme disease spirochetes"],"prefix":"10.1111","volume":"81","author":[{"given":"Jessica L.","family":"Kostick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee T.","family":"Szkotnicki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth 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