{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:41:03Z","timestamp":1791200463503,"version":"4.2.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,5,26]],"date-time":"2011-05-26T00:00:00Z","timestamp":1306368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We have characterized the <jats:italic>csoR\u2010copA\u2010copZ<\/jats:italic> copper resistance operon of the important human intracellular pathogen <jats:italic>Listeria monocytogenes<\/jats:italic>. Transcription of the operon is specifically induced by copper, and mutants lacking the P<jats:sub>1<\/jats:sub>\u2010type ATPase CopA have reduced copper tolerance and over\u2010accumulate copper relative to wild type. The copper\u2010responsive repressor CsoR autoregulates transcription by binding to a single 32\u2003bp site spanning the \u221210 and \u221235 elements of the promoter. Copper co\u2010ordination by CsoR derepresses transcription of the operon and alters CsoR:DNA complex assembly as determined by DNase I footprinting and electrophoretic mobility shift assays, with some DNA\u2010binding capacity being retained in the presence of 2 mole equivalents of copper. Analysis of the CsoR copper sensory site demonstrated that substitution of Cys<jats:sup>42<\/jats:sup> with Ala generated a CsoR variant that was unresponsive to copper. Importantly, in the absence of CopZ, copper responsiveness of <jats:italic>csoR\u2010copA\u2010copZ<\/jats:italic> expression is substantially increased, implying that CopZ reduces the access of CsoR to copper. Furthermore, CopZ is shown to confer copper resistance in mutants lacking copper\u2010inducible <jats:italic>csoR\u2010copA\u2010copZ<\/jats:italic> expression, thus providing protection from the deleterious effects of copper within the cytoplasm.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07705.x","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:39:35Z","timestamp":1305218375000},"page":"457-472","source":"Crossref","is-referenced-by-count":88,"title":["The combined actions of the copper\u2010responsive repressor CsoR and copper\u2010metallochaperone CopZ modulate CopA\u2010mediated copper efflux in the intracellular pathogen <i>Listeria monocytogenes<\/i>"],"prefix":"10.1111","volume":"81","author":[{"given":"David","family":"Corbett","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Schuler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Glenn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter W.","family":"Andrew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer S.","family":"Cavet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian 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