{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:05:04Z","timestamp":1784073904343,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,6,23]],"date-time":"2011-06-23T00:00:00Z","timestamp":1308787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:italic>Mycoplasma pneumoniae<\/jats:italic> is a wall\u2010less human respiratory tract pathogen that colonizes mucosal epithelium via a polar terminal organelle having a central electron\u2010dense core and adhesin\u2010related proteins clustered at a terminal button. A mutant lacking J\u2010domain co\u2010chaperone TopJ is non\u2010cytadherent and non\u2010motile, despite having a core and normal levels of the major cytadherence\u2010associated proteins. J\u2010domain co\u2010chaperones work with DnaK to catalyse polypeptide binding and subsequent protein folding. Here we compared features of the <jats:italic>topJ<\/jats:italic> mutant with other cytadherence mutants to elucidate the contribution of TopJ to cytadherence function. The <jats:italic>topJ<\/jats:italic> mutant was similar ultrastructurally to a non\u2010cytadherent mutant lacking terminal organelle proteins B\/C, including aberrant core positioning and cell morphology in thin sections, but exhibited a hybrid satellite growth pattern with features of mutants both having and lacking a core. Time\u2010lapse images of mycoplasmas expressing a YFP fusion with terminal organelle protein P41 suggested that terminal organelle formation\/positioning was delayed or poorly co\u2010ordinated with cell growth in the absence of TopJ. TopJ required a core for localization, perhaps involving HMW1. P1 trypsin accessibility on other non\u2010cytadherent mutants was significantly enhanced over wild type but unexpectedly was reduced with <jats:italic>topJ<\/jats:italic> mutant cells, suggesting impaired processing, translocation and\/or folding of this adhesin.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07712.x","type":"journal-article","created":{"date-parts":[[2011,6,3]],"date-time":"2011-06-03T08:54:11Z","timestamp":1307091251000},"page":"528-539","source":"Crossref","is-referenced-by-count":17,"title":["Loss of co\u2010chaperone TopJ impacts adhesin P1 presentation and terminal organelle maturation in <i>Mycoplasma pneumoniae<\/i>"],"prefix":"10.1111","volume":"81","author":[{"given":"Jason M.","family":"Cloward","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duncan C.","family":"Krause","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,6,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.12.3680-3688.2001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.151.3.1514-1522.1982"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.102.3.855-861.1970"},{"key":"e_1_2_6_5_1","first-page":"321","article-title":"Motility and multiplication of Mycoplasma pneumoniae. A phase contrast study","volume":"32","author":"Bredt W.","year":"1968","journal-title":"Pathol Microbiol (Basel)"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-1-95"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06602.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07196.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"4163","DOI":"10.1128\/iai.58.12.4163-4165.1990","article-title":"Characterization of the gene for a 30\u2010kilodalton adhesion\u2010related protein of Mycoplasma pneumoniae","volume":"58","author":"Dallo S.F.","year":"1990","journal-title":"Infect Immun"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/298765a0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1128\/iai.61.4.1523-1530.1993","article-title":"The immunodominant 90\u2010kilodalton protein is localized on the terminal tip structure of Mycoplasma pneumoniae","volume":"61","author":"Franzoso G.","year":"1993","journal-title":"Infect Immun"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.70.1.603"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-136-3-471"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.91.2.537"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.455985.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.5.1270-1276.1998"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1068408"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05507.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00867-07"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.18.6281-6289.2005"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608051103"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.9.3479-3483.1995"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1993.1047"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05113.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.15.8529835"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01344-06"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.20.6944-6955.2004"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03899.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.35.3.809-817.1982"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb13561.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.7.2906-2913.1992"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.3.974-979.1994"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.3.843-846.1995"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-3-741"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.7.2874"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-131-34061"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.144.1.390-399.1980"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00796-10"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00427.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-4-1045"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.4.1079-1087.1999"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.3.1082-1091.2003"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.5.1621-1630.2001"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.841314"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.4.697-728.2004"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.11.3061-3068.2002"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2958.2011.07712.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2958.2011.07712.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T11:05:05Z","timestamp":1697281505000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2958.2011.07712.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6,23]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["10.1111\/j.1365-2958.2011.07712.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2958.2011.07712.x","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,6,23]]}}}