{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T20:05:13Z","timestamp":1771272313997,"version":"3.50.1"},"reference-count":65,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,8,4]],"date-time":"2011-08-04T00:00:00Z","timestamp":1312416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Vibrio cholerae<\/jats:italic>, the causative agent of cholera, has an absolute requirement for iron. It transports the catechol siderophores vibriobactin, which it synthesizes and secretes, and enterobactin. These siderophores are transported across the inner membrane by one of two periplasmic binding protein\u2010dependent ABC transporters, VctPDGC or ViuPDGC. We show here that one of these inner membrane transport systems, VctPDGC, also promotes iron acquisition in the absence of siderophores. Plasmids carrying the <jats:italic>vctPDGC<\/jats:italic> genes stimulated growth in both rich and minimal media of a <jats:italic>Shigella flexneri<\/jats:italic> mutant that produces no siderophores. <jats:italic>vctPDGC<\/jats:italic> also stimulated the growth of an <jats:italic>Escherichia coli<\/jats:italic> enterobactin biosynthetic mutant in low iron medium, and this effect did not require <jats:italic>feoB<\/jats:italic>, <jats:italic>tonB<\/jats:italic> or <jats:italic>aroB<\/jats:italic>. A tyrosine to phenylalanine substitution in the periplasmic binding protein VctP did not alter enterobactin transport, but eliminated growth stimulation in the absence of a siderophore. These data suggest that the VctPDGC system has the capacity to transport both catechol siderophores and a siderophore\u2010free iron ligand. We also show that VctPDGC is the previously unidentified siderophore\u2010independent iron transporter in <jats:italic>V.\u2003cholerae<\/jats:italic>, and this appears to complete the list of iron transport systems in <jats:italic>V.\u2003cholerae<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07775.x","type":"journal-article","created":{"date-parts":[[2011,7,26]],"date-time":"2011-07-26T13:36:32Z","timestamp":1311687392000},"page":"1446-1458","source":"Crossref","is-referenced-by-count":29,"title":["The <i>Vibrio cholerae<\/i> VctPDGC system transports catechol siderophores and a siderophore\u2010free iron ligand"],"prefix":"10.1111","volume":"81","author":[{"given":"Elizabeth E.","family":"Wyckoff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley 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