{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:00:06Z","timestamp":1791115206407,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,8,30]],"date-time":"2011-08-30T00:00:00Z","timestamp":1314662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aetiological agent of Lyme disease, <jats:italic>Borrelia burgdorferi<\/jats:italic>, is transmitted via infected <jats:italic>Ixodes<\/jats:italic> spp. ticks. Infection, if untreated, results in dissemination to multiple tissues and significant morbidity. Recent developments in bioluminescence technology allow <jats:italic>in vivo<\/jats:italic> imaging and quantification of pathogenic organisms during infection. Herein, luciferase\u2010expressing <jats:italic>B.\u2003burgdorferi<\/jats:italic> and strains lacking the decorin adhesins DbpA and DbpB, as well as the fibronectin adhesin BBK32, were quantified by bioluminescent imaging to further evaluate their pathogenic potential in infected mice. Quantification of bacterial load was verified by quantitative PCR (qPCR) and cultivation. <jats:italic>B.\u2003burgdorferi<\/jats:italic> lacking DbpA and DbpB were only seen at the 1\u2003h time point post infection, consistent with its low infectivity phenotype. The <jats:italic>bbk32<\/jats:italic> mutant exhibited a significant decrease in its infectious load at day 7 relative to its parent. This effect was most pronounced at lower inocula and imaging correlated well with qPCR data. These data suggest that BBK32\u2010mediated binding plays an important role in <jats:italic>B.\u2003burgdorferi<\/jats:italic> colonization. As such, <jats:italic>in vivo<\/jats:italic> imaging of bioluminescent <jats:italic>Borrelia<\/jats:italic> provides a sensitive means to detect, quantify and temporally characterize borrelial dissemination in a non\u2010invasive, physiologically relevant environment and, more importantly, demonstrated a quantifiable infectivity defect for the <jats:italic>bbk32<\/jats:italic> mutant.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07801.x","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T06:00:50Z","timestamp":1314856850000},"page":"99-113","source":"Crossref","is-referenced-by-count":108,"title":["Bioluminescent imaging of <i>Borrelia burgdorferi in vivo<\/i> demonstrates that the fibronectin\u2010binding protein BBK32 is required for optimal infectivity"],"prefix":"10.1111","volume":"82","author":[{"given":"Jenny A.","family":"Hyde","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric 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