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TadZ is required, but its function has been elusive. We show that <jats:italic>tadZ<\/jats:italic> genes belong to the <jats:italic>parA<\/jats:italic>\/<jats:italic>minD<\/jats:italic> superfamily of genes and that TadZ from <jats:italic>A.\u2003actinomycetemcomitans<\/jats:italic> (AaTadZ) forms a polar focus in the cell independent of any other <jats:italic>tad<\/jats:italic> locus protein. Mutations indicate that regions in AaTadZ are required for polar localization and biofilm formation. We show that AaTadZ dimerizes and that all TadZ proteins are predicted to have a Walker\u2010like A box. However, they all lack the conserved lysine at position 6 (K6) present in the canonical Walker\u2010like A box. When the alanine residue (A6) in the atypical Walker\u2010like A box of AaTadZ was converted to lysine, the mutant protein remained able to dimerize and localize, but it was unable to allow the formation of a biofilm. Another essential biofilm protein, the ATPase (AaTadA), also localizes to a pole. However, its correct localization depends on the presence of AaTadZ. We suggest that the TadZ proteins mediate polar localization of the Tad secretion apparatus.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2011.07955.x","type":"journal-article","created":{"date-parts":[[2012,1,13]],"date-time":"2012-01-13T07:50:34Z","timestamp":1326441034000},"page":"694-711","source":"Crossref","is-referenced-by-count":38,"title":["The product of <i>tadZ<\/i>, a new member of the <i>parA<\/i>\/<i>minD<\/i> superfamily, localizes to a pole in <i>Aggregatibacter actinomycetemcomitans<\/i>"],"prefix":"10.1111","volume":"83","author":[{"given":"Brenda A.","family":"Perez\u2010Cheeks","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Planet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. 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